火把花根片对实验性自身免疫性脑脊髓炎大鼠脑IL-2和IFN-γ mRNA的影响

Effect of Colquhoumai root tablet on IL-2 and IFN-γ mRNA expression in rats with experimental allergic encephalomyelitis

目的:探讨火把花根片对实验性自身免疫性脑脊髓炎(EAE)Wistar大鼠的治疗作用及IL-2和IFN-γmRNA的影响。方法:模型组采用豚鼠髓鞘蛋白匀浆和福氏完全佐剂诱发大鼠急性EAE;治疗组在模型组基础上予以火把花根片(300 mg.kg-1,BID)治疗,观察症状并评分;HE染色和Loyez氏髓鞘染色,观察病理和髓鞘改变;RT-PCR法测定脑组织的IL-2和IFN-γ的mRNA表达水平。结果:治疗组未出现EAE症状,脑组织IL-2和IFN-γ的mRNA表达水平分别为0.345±0.032、0.353±0.023,与模型组比较P<0.01;脑和脊髓小血管周围炎症细胞浸润明显减少;髓鞘结构完整。结论:火把花根片对EAE有治疗作用,其机制与抑制了脑组织IL-2和IFR-γ的mRNA表达有关。

Objective： To investigate the effect of Colquhoumai root tablet on IL-2 and IFN-γ mRNA expression in experimental allergic encephalomyelitis（EAE） of rats.Methods： The allergic encephalomyelitis model was established in Wistar rats by immunization with myelin basic protein of spinal cord of guinea pig and complete Freund′sadjuvant.The rats in treatment group received Colquhoumai root tablet（300 mg·kg^-1,BID）.The symptom of EAE was observed;pathological feature and myelin of brain and spinal cord were detected with HE stain and Loyez′sstain,respectively.The expressions of IL-2 and IFN-γ mRNA were assayed by RT-PCR.Results： No EAE symptoms were developed in treatment group,the expressions of IL-2 and IFN-γ mRNA were 0.345±0.032 and 0.353±0.023,which were significantly lower than those of model group（P 〈0.01）.The histopathologic examinations revealed that less inflammation cells around vessels and demyelization in white matter of brain and spinal cords were observed in treatment group than in model group.Conclusion： Colquhoumai root tablets are effective in treatment of EAE of rats,which may be associated with inhibition of the expression of IL-2 and IFN-γ mRNA.