两种非侵入性评估红细胞成分血血容量方法的建立及应用

Development and application of two noninvasive methods for estimation of volume of red blood cell components

目的建立两种非侵入性评估红细胞成分血血容量的方法,为准确评估红细胞成分血血容量提供参考。方法检测130袋红细胞成分血的净重量及容量,计算平均血液密度,对血净重量及容量进行相关性及线性回归分析,建立回归方程;利用血液平均密度与血净重量,建立血容量计算方程。利用上述两种方程,对100袋红细胞成分血进行检测与分析,由血净重量计算出血容量作为预期值,以实际检测出的血容量作为观察值,经t检验,分析其观察值与预期值有无显著差异,以证实该方法的可靠性。结果 130袋红细胞成分血的净重量与容量呈正相关(r=0.998,P<0.01),线性回归方程为y=0.942 3 x-2.394 1(r2=0.996,d.f.=128,F=29 819.8,P<0.01);130例袋红细胞成分血的平均密度为1.072 8,血容量计算方程为y=0.932 2 x;100袋红细胞成分血血容量的预期值与观察值比较,差异无统计学意义(P>0.05)。结论已成功建立了评估红细胞成分血血容量的两种方法,该方法操作简便、安全,结果可靠,可在输血实践中推广应用。

Objective To develop two noninvasive methods and provide a reference for accurate estimation of volume of red blood cell components.Methods The net weights and volumes of 130 bags of red blood cell components were measured,based on which the mean blood density was calculated.The correlation between the net weight and volume of red blood cell components was analyzed and assessed by a linear regression model,based on which a regression equation was established.Another equation for calculation of blood volume was established based on the mean density and net weight of blood.One hundred bags of red blood cell components were tested and analyzed by the two equations,of which the volumes were calculated from net weight and used as the expected values.The difference between practically measured volumes and the expected values was analyzed by t test to verify the reliability of the method.Results The net weights of 130 bags of red blood cell components were positively related to their volumes（r = 0.998,P 〈 0.01）,of which the linear regression equation was y = 0.942 3 x-2.394 1.The mean density of the 130 bags of red blood cell components was 1.072 8,of which the equation for calculation was y = 0.932 2 x.No significant difference was observed between the practically measured volumes and the expected values of 100 bags of red blood cell components（P 〉 0.05）.Conclusion Two methods for estimation of volume of red blood cell components were developed,which were simple,safe and reliable,and were worthy of popularization in transfusion.