荣昌猪SLA-DQB基因β1结构域突变分析及蛋白质序列模式预测

Mutation Analysis and Protein Motifs Search of β1 Domain of SLA-DQB Gene in Rongchang Pigs

为了深入了解荣昌猪SLA-DQB基因β1结构域的变异及蛋白质序列模式分布情况,对53头荣昌猪SLA-DQB基因的β1结构域进行了克隆测序和序列多重比对,并在线预测蛋白质序列模式。结果发现,222bp区域内存在9个单核苷酸的插入位点、16个单核苷酸的缺失位点和89个SNPs位点。74个氨基酸中仅由SNP位点导致的氨基酸变异位点共37个,其中有24个位点氨基酸的类型发生变化。对50条SLA-DQB基因β1结构域蛋白质序列分析发现7种类型共174个蛋白质序列模式位点。单条序列中蛋白质序列模式位点最多的12个,最少的2个。蛋白质序列模式突变位点主要发生在第9、26、45、53、61个氨基酸上,涉及到5种类型蛋白质序列模式位点的改变。结果提示,荣昌猪SLA-DQB基因β1结构域存在丰富的遗传变异和多样化的蛋白质序列模式。

The study was conducted to analyze mutations and search protein motifs of β1 domain of SLA-DQB gene in Rongchang pigs.The mutation analysis of the sequences of β1 domain of SLA-DQB gene from 53 Rongchang pigs was performed by multiple sequence alignment.The protein motifs of β1 domain of SLA-DQB gene was predicted by on line softwares.The results showed that 9 single nucleotide insertion sites and 16 single nucleotide deletion sites and 89 single nucleotide polymorphism sites were observed in 222 base pairs.37 missense mutations were observed in 74 amino acid residues resulted by 37 SNPs.The change of amino acid type happened in 24 of 37 missense mutations.There were 174 protein motifs including 7 types in 50 amino acid sequences of β1 domain of SLA-DQB gene.The maximum number of protein motifs for a single sequence was 12 and the minimum number was 2.The mutations of protein motifs were observed at the 9th,26th,45th,53th and 61th amino acid residues,which involing in 5 types of protein motifs.These results indicated that β1 domain of SLA-DQB gene in Rongchang pigs owned abundant genetic diversity and diversified protein motifs.