匹格列酮抑制小鼠肝切除术后肝脏再生

Pioglitazone inhibits mouse liver regeneration induced by partial hepatectomy

目的:探讨匹格列酮在小鼠肝切除术后肝脏再生中的作用。方法:对C57BL/6J小鼠实施2/3肝切除,建立小鼠肝再生模型。实验组小鼠按体重给予匹格列酮20mg.kg-1.d-1口服,对照组给予安慰剂口服,在术后不同时间点收集小鼠残余肝脏和血清,计算肝脏体重比;监测术后肝功能和血糖变化;H&E染色观察肝脏形态学变化,免疫组化染色观察肝细胞增殖情况。结果:匹格列酮20mg.kg-1.d-1对小鼠术后肝功能和血糖无明显影响。与对照组相比,匹格列酮组小鼠术后肝脏生长缓慢,肝细胞增殖受到抑制(P<0.05)。结论:匹格列酮抑制小鼠肝切除术后肝脏再生。

AIM： To investigate the effects of pioglitazone on mouse liver regeneration induced by partial hepatectomy. METHODS： 2/3 partial hepatectomy was performed in C57BL/6J mice. The experimerltal group mice were treated with pioglitazone （20 mg·kg^-1·d^-1） by oral ga- vage. The control group mice were treated with vehicle. Mouse serum and liver tissue harvest were performed at serials time point post operation. The liver/body weight ratio were calculat- ed; liver histology was assessed by H＆E staining; serum transaminase and glucose were de- termined by biochemistry assay. Hepatocellular proliferation was eveluated by IHC staining ofKi67 and PH3. RESULTS：There were no hepatic toxic and side effects caused by pioglitazone under the given dosage. Pioglitazone didn＇t impair the metabolism of serum glucose. The liver growth and hepatocyte proliferation were delayed and inhibited in pioglitazone treated mice in comparison to that in control mice（P〈0.05）. CONCLUSION： Pioglitazone inhibits liver regeneration following partial hepatectomy in mice.