大蒜素对不同年龄组小鼠血清神经递质5-羟色胺和β-内啡肽的影响

Effects of garlicin on 5-HT,β-EP of mice at the different age groups

目的:探讨大蒜素对不同年龄组小鼠神经递质5-羟色胺(5-HT)和β-内啡肽(β-EP)的影响。方法:健康ICR小鼠60只,随机分为青年组和老年组;各组又分设对照组和实验组。老年组鼠是用D-半乳糖制备衰老模型。实验组喂食大蒜素14d,对照组灌服同体积生理盐水。采用ELISA法测各组小鼠血清5-HT和β-EP含量。结果:老年组鼠的β-EP明显低于青年组(P<0.05),而老年组鼠和青年组鼠5-HT无统计学差异(P>0.05)。青年实验组鼠较青年对照组鼠的5-HT,β-EP水平明显增加(P<0.05);老年实验组较老年对照组的β-EP水平明显增加(P<0.05),而5-HT仅有增加趋势,但差异无统计学意义(P>0.05)。结论:大蒜素对β-EP的表达有明显的上调作用,对5-HT的上调作用青年组较老年组明显。

AIM： To explore the effects of garlicin on 5-HT, β-EP of mice at the different age groups. METHODS： The mice were randomly divided into young group and senium group, and each group consists of treatment group and control group. Aging models of mice were with D-galactose. The mice in treatment group were administered orally with garlicin for consecutive 14 days and two other groups with equal amount of normal saline. The content of 5- HT and β-EP were detected by enzyme-linked immunosorbent assay （ELISA）. RESULTS： The β-EP content were lower in the senium groups those that in young groups （P 〈 0.05）. The difference of 5-HT was not signifi cant betweensenium group and young group（P〈0.05）. Garlicin improved the levels of 5-HT and β-EP in the young treatment groups （P 〈0.05）. Compared with Senium control group, the level of ^-EP was higher in senium treatment group （P 〈0.05）, but the difference of 5-HT was not signifi cant between the two groups （P 〈 0.05）. CONCLUSION. Garlicin can improve the level of β-EP of mice, and it can improve the level of 5- HT of young mice.