血IL-10、IL-5、IL-2、IFN-γ水平在反复腹痛患儿及其家庭成员中的比较

Plasma Level of IL-10,IL-5,IL-2 and IFN-γ in the Family Members of Children with Recurred Abdominal Pain

目的了解血细胞因子IL-10、IL-5、IL-2、IFN-γ水平在反复腹痛患儿及其家庭成员中的变化.方法采集3～14岁16名反复腹痛患儿及89名家庭成员血液标本3 mL,应用ELISA试剂盒完成IL-10、IL-5、IL-2、IFN-γ的测定.根据有无腹痛分为腹痛患儿组、腹痛家庭成人组、无腹痛成人组.结果血IL-10和IL-5水平在腹痛患儿组和腹痛家庭成人组中低于无腹痛家庭成人组(F10=26.77,P<0.01;F5=23.92,P<0.01).IL-2和IFN-γ水平在腹痛患儿组和腹痛家庭成人组中高于于无腹痛家庭成人组(F2=25.03,P<0.01;FIFN-γ=21.50,P<0.01).结论在腹痛患者血中IL-10和IL-5水平低提示其抗炎能力减弱(Th2功能减弱),IL-2和IFN-γ水平高提示其促炎症反应活跃(Th1功能强).

Objective To understand the level of IL-lO, IL-5, IL-2 and IFN- y in the family members of children with recurred abdominal pain. Methods The venous EDTA blood specimen （3 mL/one） were obtained from 89 family members of 16 children under 3 to 14 years old with recurred abdominal pain for investigating the level of IL-IO, IL-5, II,-2 and IFN-＇y by ELISA. The family members were divided into three groups： children with recurrent abdominal pain, family adult members with recurrent abdominal pain and adults without recurrent abdominal pain. Results The levels of IL-IO and IL-5 were lower in family children and adult members with recurrent abdominal pain than adults without recurrent abdominal pain （ （F]0 = 26.77, P 〈 0.01; Fs = 23.92, P 〈 0.01 ）. The levels of IL-2 and IFN-y were higher in in family children and adult members with recurrent abdominal pain than adults without recurrent abdominal pain （ （F2 = 25.03, P 〈 0.01 ; FIFN- ＂,/ = 21.50, P 〈 0.01） ）. Conclusion The lower levels of IL-10 and IL-5 in family children and adult members with recurrent abdominal pain suggest the poor anti-inflammtory abilities （poor Th2 function）. The high levels of IL-2 and IFN- ~/in family children and adult members with recurrent abdominal pain suggest the productive and active inflammtory responses （productive Th 1 function）.