大蒜新素对变应性鼻炎患者外周血白介素4和γ干扰素的影响

Effect of Garlicin on Serum IL-4 and IFN-γ in Allergic Rhinitis Patients

目的研究大蒜新素对变应性鼻炎患者外周血白介素4(interleukin,IL-4)和γ干扰素(interferon,IFN-γ)的影响.方法 66例研究对象均来自云南省中医医院,变应性鼻炎患者44例,其中男21例,女23例,年龄22～48岁(中位数36岁),均经变应原皮肤点刺试验确诊,变应性鼻炎患者被分为药物治疗组和阳性对照组,各22例.药物治疗组患者口服大蒜新素40 mg,4次/d,共14 d;阳性对照组患者口服等剂量的淀粉胶囊,时间、频次相同.阴性对照组22例,其中男10例,女12例,年龄20～42岁(中位数33岁),每日口服大蒜新素40 mg,4次/d,共14日.实验前与实验结束后各抽取外周静脉血5 mL,分离出血浆,用酶联免疫吸附试验(ELISA)检测血浆中IL-4和IFN-γ的浓度.结果药物治疗组、阳性对照组、阴性对照组分别有3例、3例、4例退出实验;药物治疗组、阳性对照组、阴性对照组服实验前、后血浆中IL-4的浓度(pg/mL)分别为53.20±10.10、51.94±10.40、35.67±5.69和35.87±8.82、50.27±9.68、33.53±4.86;血浆中IFN-γ的浓度(pg/mL)分别为45.66±9.43、46.04±10.87、65.11±10.93和57.01±13.34、45.64±8.90、71.07±8.51.实验前,药物治疗组与阳性对照组患者血浆IFN-γ和IL-4水平均无显著性差异(P>0.05),分别与阴性对照组比较,变应性鼻炎患者血清中IL-4水平明显高于阴性对照组,而IFN-γ水平明显低于阴性对照组,均有显著性差异(P<0.05).实验后,药物治疗组血浆IL-4水平明显低于阳性对照组,而IFN-γ水平明显高于阳性对照组,但仍低于阴性对照组,均有统计学意义(P<0.05),药物治疗组IL-4水平接近阴性对照组,无显著性差异(P>0.05).药物治疗组实验后IL-4水平较实验前明显下降,而IFN-γ水平较实验前明显升高,均有显著性差异(P<0.05).阴性对照组实验后血清IFN-γ水平有所升高,有显著性差异(P<0.05),而IL-4水平稍有下降,无统计学意义(P>0.05).阳性对照组患者血浆IL-4水平和IFN-γ水平实验前、后无明显变化.结论大蒜新素能明显改善变应性鼻炎患者的临床症状,其作用机制可能是通过降低血浆中IL-4的浓度,同时升高IFN-γ水平,恢复Th1和Th2细胞因子的平衡.

Objiective To investigate effect of garlicin on serum IL-4 and IFN-± in peripheral blood in allergic rhinitis patients. Methods All subjets were sixty people who came from Department of Otorhinolaryngology Head and Neck Surgery, the Hospital of Traditional Chinese Medicine of Yunnan Province. Among them, forty-four allergic rhinitis patiets （male 21, female 23, age range from 22 to 48 years old, median 36 years old） who were diagnosed by the allergen skin prick test were randomly divided into two groups ： pharmaceutical group and positive control group, 22 in each group. 22 subjects without atopic disease were selected into negative control group （male 10, female 12, age range from 20 to 42 years old, median 33 years old）. 40mg of capsule of garlicin was taken for each patient in pharmaceutical group and negative control group and 40 mg of capsule of farina for positive control group, orally, four times per day, 14 days totally. At the beginning and the end of study, 5 mL of peripheral blood was extracted from each patients for collecting plasma. Enzyme linked immunosorbent assay （ELISA） was utilized to detect the serum levels of IL-4 and IFN- y. Results Three patients in pharmaceutical group , three patients in positive control group and four people in negative control group retreated from this study. In pharmaceutical group, positive control group and negative control group, before and after this study, the serum levels of IL-4 were （53.20 ± 10.10） pg/mL, （51.94 ± 10.40） pg/mL, （35.67 ± 5.69） pg/mL and （35.87 ± 8.82） pg/mL, （50.27 ± 9.68） pg/mL, （ 33.53 ±4.86） pg/mL, respectively, and those of IFN-y were （45.66 ± 9.43） pg/mL, （46.04 ± 10.87） pg/mL, （65.11 ± 10.93） pg/mL and （57.01 ± 13.34） pg/mL, （45.64 ± 8.90） pg/mL, （71.07 ± 8.51） pg/mL, respectively. Before investigation, there were no significant difference in the serum levels of IL-4 and IFN- y between pharmaceutical group and positive control group. The serum levels of IL-4 in allergic rhinitis patients were higher than those in negative control group, while the serum levels of IFN- y were lower, and there were significant difference for them in negative control group compared with pharmaceutical group and positive control group, respetively. After study, the serum levels of IFN- y were higher in pharmaceutical group than those in positive control group, while the levels of IL-4 were lower, there were significant difference for them between pharmaceutical group and positive control group. The serum levels of IL-4 in pharmaceutical group after treatment were notably declined and the levels of IFN- y were markedly increased, and there were significant difference compared with before treatment. There was no notable variation in the serum levels of IFN- y and IL-4 in positive control group patients after study compared to before study. In negative control group, the serum levels of IL-4 after study were slightly declined, and serum levels of IFN- y after study were increased notably, there was significant difference compared with before study. Conclusions Garlinci could ameliorate clinical symptoms of allergic rhinitis patients and increase serum level of IFN- y and decrease serum level of IL-4 markedly in allergic rhinitis patients.It plays an important role in regulating serum levels of cytokines of Thl and Th2.