KOH醇水溶液分离纯化结构脂质条件研究

Purification of structured lipids by KOH-hydroalcoholic solution

以酶催化酸解制备MLM型(M为中碳链脂肪酸,L为长碳链脂肪酸)结构脂质得到的反应产物为研究对象,探讨了KOH醇水溶液中和脱除游离脂肪酸条件中KOH醇水溶液用量、醇-水体积比、正己烷用量以及二次提取的正己烷用量对结构脂质回收率及组成的影响。结果表明:KOH醇水溶液与反应产物体积(mL)质量(g)比为8∶1,醇水溶液中醇-水体积比为30∶70,正己烷与反应产物体积(mL)质量(g)比为12∶1;二次提取中正己烷与反应产物体积(mL)质量(g)比为3∶1时,体系中结构脂质回收率最高,为94.35%;回收得到的结构脂质中甘油三酯含量为95.27%,结构脂质酸值(KOH)为0.11 mg/g。

Reaction products obtained from enzymatic aeidolysis preparation of MLM -type （M, medium - chain fatty aeids ;L,long- ehain fatty acids） structured lipids （SLs）were studied. The effects of KOH - hydroaleoholie solution to remove free fatty aeids on reeovery rate and composition of SLs were discussed, such as the volume of the KOH - hydroaleoholie solution, the ethanol - water ratio of the solution, the volume of the hexane and the volume of the hexane in second extraction step. The results indicated that the highest recovery rate was attained with the proportion of KOH -hydroaleoholie solution volume（mL） and reaction products mass（g） 8： 1, ethanol and water volume ratio 30： 70, hexane volume（mL） and reaction produets mass （g） ratio 12： 1, in second extraction step hexane volume （mL） and reaction products mass （g） ratio 3： 1. Under these conditions the recovery rate of SLs was 94. 35%, and triaeylglyeerol content and the acid value of SLs were 95. 27% and 0. 11 mg （KOH）/g, respectively.