摘要
目的探讨原发性肝细胞癌(Hepatocellular Carcinoma, HCC)中 Sonic Hedgehog(Shh)信号通路基因和β-catenin的表达情况及其临床意义。方法采用免疫组织化学方法对 49 例 HCC 和相应癌旁肝组织中Gli1和β-catenin蛋白表达,荧光定量PCR检测2个肝癌细胞系和正常肝细胞中相应基因mR-NA的表达情况。结果免疫组化染色与 RT-PCR 示 Gli1 在 HCC 组织中的阳性表达率为 77.56%,而癌旁肝组织为40.82%,两者差异具有显著意义(P < 0.05)。β-catenin蛋白在HCC中的表达显著高于癌旁肝组织(P < 0.05),且与Gli1的表达呈正相关(r = 0.594,P = 0.000)。Gli1和β-catenin mRNA在肝癌细胞株中高表达,在正常肝细胞中低表达(P < 0.05)。结论 Gli1 和β-catenin 在肝癌细胞中均过度表达,可能协同作用促进肝癌细胞异常增殖,参与HCC的发生和发展。
Objective To study the expression of Glil in HCC and its relationship with the expression of β-catenin. Methods The protein and mRNA expression of Glil were detected by immunohistocbemistry and RT-PCR respectively in 49 cases of HCC tissues and corresponding adjacent liver tissues. Results The positive staining rate of Glil in HCC tissues was significantly higher than in the adjacent tumor tissues （77.56% vs 40.82%, P 〈 0.05）. The expression of β-catenin in HCC was higher than in adjacent tumor tissues（69.39% vs 51.02%）. There was a significant positive correlation between the expression of Glil and β-catenin（r = 0.594, P = 0.000）. Conclusion The over-expression of Glil may play a crucial role in occurrence and progress of HCC by upregulating β-catenin, which leads to the abnormal proliferation of HCC cells.
出处
《现代消化及介入诊疗》
2012年第4期185-188,共4页
Modern Interventional Diagnosis and Treatment in Gastroenterology
基金
广东省自然科学基金(10451051501005740)
南方医科大学附属南方医院院长基金(2008C025)
