微柱凝胶法和传统试管法在新生儿溶血病诊断中的比较研究

Application of microcolumn gel technology in diagnosing hemolytic disease of the newborn

目的:探讨微柱凝胶技术对新生儿溶血病(HDN)的诊断价值。方法:选取128例疑似HDN的新生儿,分别用微柱凝胶法和传统试管法进行直接抗人球蛋白试验(DAT)、间接抗人球蛋白试验(IAT)和放散试验并对比结果。结果:在128例中104例诊断为HDN患儿,微柱凝胶法的阳性检出率为92.31%,传统试管法为80.77%,微柱凝胶法的敏感性高于传统试管法(χ2=5.92,P<0.05);在微柱凝胶法3项试验中,以放散试验的阳性率最高,DAT阳性率最低。结论:对于辅助诊断HDN,微柱凝胶法比传统试管法更具优势。

Objective ： To explore the value of the microcolumn gel technology in diagnosing hemolytic disease of the newborn. Methods ： The direct antiglobulin test （ DAT）, indirect antiglobulin test （IAT） and elution test were conducted with the microcolumn gel technology and the traditional method in 128 newborns suspected hemolytic disease of the newborn. Results： In 104 newborns with clinical diagnosis of hemolytic disease of the newborn, the positive rates of the microcolumn gel technology and the traditional method were 92.31% and 80.77% （X2 = 5.92, P 〈 0.05）. As for three methods with the microcolumn gel technology, the positive rate of the elution test was the highest and that of DAT was the lowest. Conclusion： A microcolumn gel technology is better for assistant diagnosis of hemolytic disease of the newborn when compared with the traditional method.