HPLC测定枣仁安神液中的五味子醇甲

HPLC determination of schisandrin in Zaoren Anshen oral solution

目的:建立枣仁安神液中五味子醇甲的HPLC含量测定方法。方法:SupelcosilTMLC-18-DB ODS(150 mm×4.6 mm,5μm)分析柱,流动相为甲醇-水(60∶40),流速1.0 mL.min-1,检测波长250 nm。结果:浓度在0.32～1.28μg范围内与色谱峰面积呈线性关系(r=0.9999),平均加样回收率96.6%(n=9)。结论:方法简便、快速,精密度和稳定性良好,适合于枣仁安神液生产的质量控制。

Objective：To establish an HPLC method for schisandrin in Zaoren Anshen oral solution.Methods：SupelcosilTM LC-18-DB ODS（150 mm×4.6 mm,5 μm） column was adopted as analytical column with mobile phase of methanol-water（60∶ 40） at the flow rate of 1.0 mL·min-1,and detective wavelength was 250 nm.Results：The standard curve was good linear in the range of 0.32-1.28 μg（r=0.9999）,and the average recovery was 96.6%（n=9）.Conclusion：The HPLC method is convenient,quickly,selective and accurate,which can be used for quality control for Zaoren Anshen oral solution.