摘要
根据NCBI上公布的猪Lp-PLA2mRNA序列设计引物,以小型猪肺脏cDNA为模板进行PCR,将扩增产物连接进入具有EF1α启动子的pIRES2-EGFP真核表达载体中,并对其功能进行鉴定。然后,将猪Lp-PLA2表达载体转染入小型猪胎儿成纤维细胞,经过G418抗性筛选,鉴定,成功得到5株转Lp-PLA2基因的猪成纤维细胞系。这为下一步建立Lp-PLA2转基因猪及其功能研究奠定基础。
The primer of this study was designed according to Lp-PLAz mRNA sequence of procine published by NC- t3I. Take small pig lung cDNA as template for PCR,then link it to plRES2-EGFP eukaryotic expression vector with the promoter of EFla and identify the function of the compound. Afterwards, transfect it into fetus fibroblasts of small pig, selecting and evaluating with the resistant of G418, we successfully get 5 strains fiber cells of pig with gene Lp-PLAz. This study lays a foundation for the further construction of Lp-PLAz transgenic pig and provides a new i- dea for the research on Lp-PLAz function.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2012年第8期1164-1168,共5页
Chinese Journal of Veterinary Science
基金
国家"973"计划重大科学问题导向资助项目(2011CBA01003)
