压力蒸汽灭菌后无菌包的有效期探讨

Experimental study on period of validity of sterilizing packages after high pressure sterilization

目的探讨棉布包装的各种灭菌包,经预真空高压蒸汽灭菌后在不同季节的有效期。方法选取2011年1、8月,共两批次,每次用双层棉布包装的100个无菌包,经预真空高压蒸汽灭菌后,按编号分为10组,存放在有空气调节系统的10间病房治疗室无菌物品专用柜内,所有无菌包分别在保存第6、10、14、18、22天取样进行细菌培养。结果随机抽取10间治疗室中的两间,共20个无菌包外层与内层包布间及包内物品中放置的沙条做细菌培养,两批次,80份细菌培养标本全部无菌生长,结果均为阴性。结论无菌包在达到包装、消毒、存放基本要求的基础上,放置于有空气调节系统的病房治疗室无菌物品专用柜内,可以不考虑季节和天气的影响,将有效期延长为14d。

OBJECTIVE To explore the period of validity for various sterilizing packages in different seasons after pre-vaccum high pressure sterilization. METHODS We finished our experiments separately in Jan and Aug, 2011 for the biggest wheather change in these two months. In each experiment, packing incision of trachea packs, vein incision packages, rescue packages, urethral catheterization packages, suture packages, totally 100 packages were divided into 10 groups. After pre-vaccum high pressure sterilization, the packages were stored in sterile special cabinet of treatment rooms in 10 different wards, 10 packages in each treatment room, All the packages were sampled for bacterial culture on the 6th, 10th, 14 th , 18th and 22nd of the storage. RESULTS At 9 am on 6th, 10th, 14th, 18th, and 22th days after vaccum pressure sterilization, we randomly selected two packages from each treatment room, totally 20 packages. Under non-bacterial condition in bacterial cultural rooms, the gauzes between two layers of packing clothes and the guazes inside the package were cultured in two groups, the results showed negative, there was no bacterial growth in all the samples. CONCLUSION If sterilizing packages meet the basic requirements including packing, sterilization, the period of validity of the packages stored in the wards with air conditioner system can be extended to 14 days without the influence of the seasons and weather.