细菌海洋酶提取胶原蛋白的工艺优化

Enzymolysis technology optimization on the extraction of collagen from Greenland halibut skin

胶原蛋白是一种营养丰富应用广泛的功能蛋白。以单因素试验为基础,以鱼皮胶原蛋白提取率为指标,采用正交试验对酶用量、酶解温度、酶解pH、酶解时间进行优化,探讨了酶法提取黑鲽鱼皮胶原蛋白的新工艺。结果表明:细菌海洋酶的提取率优于其他蛋白酶;当酶剂量为1.0%、酶解时间120min、酶解温度55℃、pH9.0时,鱼皮的胶原蛋白提取率最佳,为26.1%。

Collagen was a kind of structural proteins with abundant nutrition and biological function used in food and medicine. Based on orthogonal experiment and single factor experiment, using collagen extraction rate from Greenland halibut skin as index, enzyme amount, enzymolysis temperature, enzymolysis pH and enzymolysis time were optimized. The results indicated that the extraction rate by marine protease was superior to other protease extracted from animal organs, and the extraction rate 26.1% is the highest under the condition of enzyme amount 1.0%, enzymolysis duration 120 min, temperature 55 ℃, pH9.0.