摘要
观察了枯草杆菌α-淀粉酶、蛋白酶在聚乙二醇/硫酸铵和聚乙二醇/磷酸盐两个双水相系统中的分配行为。设计了借液-液萃取方式分别部分纯化上述两酶粗品的方案。经部分纯化后α-淀粉酶的比活力(按每毫克制品含活力单位数计算)提高至6倍,活力回收率可达80%;蛋白酶比活力提高至8倍,活力回收率在50～60%间。此法具有简易、快速、有效、并可准确放大缩小操作规模等特点。
The partition characteristics of B.sublilis′ α-amylase and proteinase in PEG 6000/(NH_4)_2SO_4 and PEG6000/sodium phosphates(pH7.5)aqueous two-phase systems were observed.The factors that influence the distribution coefficients and stability of the enzymes were investigated.Procedures of liquid liquid extraction were developed for the partially purification of the crude preparations of B.sublilis′α-amylase and Proteinase.After partially purifying the specific activity(units/mg preparation)of α-amylase and proteinase preparations raised to 8 times and 6 times respectively,and the recovery of the two enzymes activity were 80% and 60% respectively.These procedures were very easy to operate,high speed,and effi-
cacious,and their operative scale can be amplified or reduced without changing efficiency.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
1990年第2期102-110,共9页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家自然科学(高技术)基金~~
关键词
枯草杆菌
α-淀粉酚
双水相系统
aqueous two-phase systems
partially purification
Bacillus subtilis'α-amylase
proteinase
