蒙古族Alzheimer病患者载脂蛋白E基因多态性研究

Study on the polymorphism of apolipoprotein E in Mongolian patients with Alzheimer's disease

目的探讨载脂蛋白E(ApoE)基因多态性与蒙古族Alzheimer病(AD)的关系。方法用聚合酶链反应及基因测序技术检测106例蒙古族AD患者和100名正常对照者的ApoE基因的基因型和等位基因频率,并进行比较。结果 AD组中有ApoEε4基因型频率(31.1%)显著高于正常对照组(17.0%)(χ2=5.591,P=0.018;OR=2.207,95%CI:1.136～4.289);ε4等位基因的频率显著高于正常对照组(χ2=4.27,P=0.039;OR=1.841,95%CI:1.026～3.304);两组ApoEε2、ε3等位基因频率的差异无统计学意义。结论ApoE基因ε4等位基因可能是蒙古族人群患AD的遗传性危险因素。

Objective To investigate the relationship between apolipoprotein E （ApoE） gene polymorphism and Alzheimer＇s disease （AD） in Mongolian population. Methods ApoE genotype and allelic genes frequency of 106 Mongolian AD patients and 100 Mongolian normal controls were detected by applying polymerase chain reaction （PCR） and gene sequencing technology, and the results were compared. Results The ratio of ApoE ε4 genotype in AD group （31.1% ） was significantly higher than that in normal control group （17.0%） （X^2 = 5. 591, P = 0. 018; OR =2. 207, 95% CI：I. 136 -4. 289）. Frequency of ApoE gene e4 allele in AD group was significantly higher than that in normal control group （X^2 = 4.27, P = 0. 039 ; OR = 1. 841,95% CI： 1. 026 - 3. 304）. The difference of ApoE ε2 and ε3 allele between the two groups was not statistically significant. Conclusion Carrying ε4 allele gene of the ADoE mav be a genetie risk factor of AD to Mongols.