应用细菌培养法与PCR检测法检测淋病奈瑟氏菌的对比研究

Neisseria gonorrhoeae, bacterial culture and PCR results

目的:比较用PCR(聚合酶链反应)检测法和细菌培养法检测淋病奈瑟氏菌的效果。方法:同时用PCR检测法和细菌培养法对126例疑似淋病患者的生殖道分泌物进行检测。待检测结果出来后,对使用两种检测法测得的阳性检测结果进行比较分析。结果:使用PCR检测法测得的淋病奈瑟氏菌阳性率为76.98%,用细菌培养法测得的淋病奈瑟氏菌阳性率为44.44%。用PCR检测法测得的淋病奈瑟氏菌阳性检出率明显高于用细菌培养法测得的这一结果,且两者间的差异具有显著性(P<0.05)。结论:用PCR检测法检测淋病奈瑟氏菌具有特异性强、灵敏度高、漏诊率低等优点,不失为一种检测淋病奈瑟氏菌的好方法。

Objective PCR （polymerase chain reaction） assay the effect of the detection of Neisseria gonorrhoeae by bacterial culture. Methods Genital tract secretions of 126 cases of suspected gonorrhea patients were PCR assay and bacterial culture detection, a comparative analysis of the positive test results of the two methods. Results PCR detection of Neisseria gonorrhoeae-positive rate of 76.98%, the bacteria Neisseria gonorrhoeae culture positive rate was 44.44%. The PCR assay was positive rate was significantly higher than the culture method. Comparison two methods have statistically significant difference （P 0.05）. Conclusions： PCR assay was specific, more targeted, higher sensitivity and low rate of missed diagnosis can be used as the preferred method for detection of Neisseria gonorrhoeae in the chronic gonorrhea.