溶血磷脂酸对子宫内膜癌RL-952细胞尿激酶型纤溶酶原激活剂分泌的影响

The effect of urokinase-type plasminogen activator secretion on endometrial carcinoma RL-952 cell by stimulating of lysophosphatidic acid

目的探讨溶血磷脂酸(lysophosphatidic acid,LPA)对子宫内膜癌RL-952细胞尿激酶型纤溶酶原激活剂(urokinase-type plasminogen activator,uPA)分泌的影响。方法不同浓度的LPA刺激子宫内膜癌RL-952细胞24h,80μmol/LLPA刺激子宫内膜癌RL-952细胞不同时间后收集细胞上清液,采用酶联免疫吸附试验检测uPA的分泌情况,Transwell试验检测LPA对子宫内膜癌RL-952细胞体外迁移/侵袭能力的影响。结果 2、10μmol/L的LPA即可诱导子宫内膜癌RL-952细胞uPA的分泌增加,且随着LPA浓度的加大,uPA分泌量明显增多,二者呈明显的浓度依赖关系;80μmol/L的LPA作用于RL-952细胞时,随着时间的延长uPA分泌量增多,二者呈明显的时间依赖关系。并且LPA可以明显增加子宫内膜癌RL-952细胞的体外迁移/侵袭能力。结论 LPA可成为子宫内膜癌早期诊断及预后监测的分子标志物,并有望成为子宫内膜癌分子靶向治疗的靶标。

Objective To investigate the effect of urokinase-type plasminogen activator（uPA） secretion on endometrial carcinoma RL-952cell by stimulating of lysophosphatidic acid（LPA）.Methods Supernatant of endo-metrial carcinoma RL-952 cells was collected after stimulating with determined concentrations of LPA for 24hours or with 80μmol/L LPA at determined times,levels of urokinase-type plasminogen activator（uPA）in these super-natant were determined by enzyme labeled immunosorbent assay（ELISA）.Transwell chamber assay was used to detect the migration/invasion abilities.Results 2μmol/L,10μmol/L concentrations of LPA can induce uPA secretion,and with the concentration increasing,the uPA production increased significantly which showed obvious con-centration-dependent,and also it showed significant time-dependent.The LPA significantly increased the migration/ invasion of endometrial cancer RL-952cells.Conclusions LPA is expected to become the molecular markers,the LPA receptor and its signaling pathway is expected to become the target of the molecular therapy of endometrial cancer.