荷瘤小鼠脾脏过度增大的机制

Mechanism of Splenic Excess Augmentation in Tumor-bearing Mice

目的研究H22荷瘤小鼠生长过程中脾脏过度增大的机制。方法通对小鼠脾指数与脾脏细胞总数进行相关性分析、检测脾细胞生长周期以及T、B淋巴细胞比例。结果小鼠脾指数与脾脏细胞总数呈显著正相关性(r=1.000,P<0.01);H22荷瘤小鼠与对照组小鼠脾脏细胞的细胞周期无明显差异;H22荷瘤小鼠与对照组小鼠相比脾脏总T淋巴细胞、CD8+T与CD4+T细胞比例增大(P<0.05),B细胞比例基本不变,CD4+/CD8+减小。结论 H22荷瘤小鼠脾脏增大与各类免疫细胞转移至脾脏后滞留有直接的关系,其中CD8+T细胞的积累最显著,脾脏的免疫抑制作用与CD8+T细胞有密切的关系。

Objective To explore the mechanism of splenic excess augmentation in H22-beared mice.Methods Correlation of splenic index and cell count was analyzed.Splenic cell cycle and the variety of lymphocyte were examined by flow cytometry（FCM）.Results Significant correlation was observed between splenic index and total cell number（r=1.000,P〈0.01）.There was no obvious diversity in splenic cell cycle of mice.The proportion of T cell,CD8＋T cell and CD4＋T cell increased（P〈0.05）.At the same time CD4＋/CD8＋declined.There was no marked change in the proportion of B cell.Conclusion The excess augmentation in H22 bearing mice was associated with the export of cells which was restrained after the cells entered the spleen,especially the CD8＋T cell was detected.CD8＋T cell was bound up with immunosuppression of spleen in H22-beared mice.