结缔组织生长因子在周围神经慢性卡压损伤中的表达及其意义

Expression and role of connective tissue growth factor in the peripheral nerve after chronic compres-sion injury

目的探讨大鼠坐骨神经慢性卡压损伤后其卡压神经段内结缔组织生长因子（CTGF）表达变化及其意义。方法2010年7月到2010年9月，将50只成年雄性AD大鼠随机分成：A组（假手术组），仅分离暴露坐骨神经；B组（卡压组），采用Mackinnon建立的坐骨神经卡压模型方法对大鼠右侧后腿坐骨神经行硅胶管卡压术。于卡压术后2、4、6、8、10周时间点，随机取A、B组大鼠各5只，取其卡压段坐骨神经行组织形态学、免疫组织化学观察及RT-PCR、Westernblot定量测定CTGF及Ⅰ、Ⅲ型胶原蛋白（COL-Ⅰ、Ⅲ）含量。结果坐骨神经慢性卡压损伤后，有髓神经纤维发生瓦勒变性，胶原纤维增生．CTGF表达上调，并伴有COL-Ⅰ、Ⅲ表达升高，与假手术组比较差异有统计学意义（P〈0．05）。结论慢性卡压损伤可致周围神经发生纤维化病变，CTGF参与了这一病理生理改变过程，提示CTGF在周围神经纤维化过程中具有一定作用。

Objective To investigate the effects of connective tissue growth factor （CTGF） on the chronic peripheral nerve compression injury and explore the function of CTGF in peripheral nerve compression injury and repair. Methods From July 2010 to September 2010, fifty adult male SD rats were randomly divided into group A and B： group A （sham-operated group）： only exposed the sciatic nerve; group B （compression group）： undergone sciatic nerve entrapment operation on the right hind leg according to the method which Mackinnon adopted when he established the model of chronic sciatic nerve compression. Elec- tron microscopy, immunohistochemistry, RT-PCR and Western-blot were performed to observe the morpholog- ical changes of the compressed nerve tissue and to determine the level of CTGF, collagen- Ⅰ , Ⅲ （COL- Ⅰ , Ⅲ ）, 2, 4, 6, 8, 10 weeks after the surgery, respectively. Results After sciatic nerve compression, the collagen in nerve increased; The expression of CTGF and COL- I , III in sciatic nerve of compressed group increased, which was statistically different compared with the sham-operation group （P 〈 0.05）; In the meanwhile, the contents of CTGF and COL- Ⅰ , Ⅲ were positively correlated in a certain period. Conclu- sion Peripheral nerve fibrosis can be caused by chronic nerve compression. The expression of COL- Ⅰ , Ⅲ in sciatic nerve increased and CTGF get involved in the pathophysiological process, which suggests that CTGF plays an important role in the process of neural injury and fibrosis.