体外细胞组织重建中共培养技术的应用

The application of cell co-culture in the reconstruction of cells and tissues in vitro

细胞共培养体系在维持细胞基本结构和性状的基础上,通过两种或多种细胞组织的相互作用,使体内外环境尽可能相吻合,弥补了单层细胞培养的缺陷,有利于构建更加接近生理状态的重建体外细胞组织,被广泛应用于现代细胞研究,成为角膜、牙周、软骨、心血管以及神经细胞组织等体外构建的重要技术应用。在干细胞研究中,多孔膜两侧直接接触共培养日益受到重视,三维细胞共培养将是未来发展的方向。

Based on maintaining the fundamental structures and properties of cells, the cell co-culture system makes the environment in vitro to be similar with in vivo environment as far as possible through interactions of two or more kinds of cells and tissues. So the cell co-culture system makes up for the defects of monolayer cell culture and is conducive to reconstructing cells and tissues in vitro closer to the physiological state. The system is widely used in modern cell research, which makes it to be an important technology for the cells and tissues reconstruction in vitro of cornea, periodont, cartilage, cardiovascular, neural, et al. In the field of stem cell research, the co-culture sys- tem of direct contact through porous membrane has been paid much attention recently and three-dimensional culture will be the focal point in the future.