MAR介导的非病毒附着体载体的研究进展

Research Progress of the Matrix Attachment Mediated Nonvirus Episomal Vector

附着体载体可以使外源基因在宿主细胞中不整合到基因组上而是以附着体的形式存在,是一种新型的高效、安全、附着体表达载体。目前研究较多的是由S/MAR(Scaffold/Matrix Attachment Region,S/MAR)元件介导的质粒。虽然此类载体均能介导载体附着存在,但是转基因的表达量不同。最近研究发现,载体的启动子、骨架结构、CpG基序以及表达系统等方面能够影响转基因的表达,针对以上内容作一综述,希望据此进一步优化载体,为临床研究提供基础依据。

The new type of episomal expression vectors can make transgene expression efficient, continuous, and safety, which do not integrate into the genomes and exist in form of episome in host cells. Currently, more researches focus on episomal vectors which are mediated by scaffold/matrix attachment region components. Though episomal vectors exist in form of episome, there are many differents in the terms of transgene expression. It was found that many factors were affected on transgene expression based on the recent researches, for example, the promoter, the skeleton structure, CpG sequence of the vector, and the express system. Here the development of MAR madiated transgene expression was reviewed. According to it, it is better to optimize the vector furtherly and provide the basis for clinical researches.