CYP4F2基因单核苷酸多态与缺血性脑卒中的相关性

The Association of Polymorphisms of CYP4F2 Gene with Ischemic Stroke

目的 探讨CYP4F2基因多态与中国北方汉族人群缺血性脑卒中的相关性。方法采用病例-对照研究，检测CYF4F2基因rs2108622、rs3093100、rs3093105、rs3093135、rs1558139位点，以及该基因rs2108622-rs3093100-rs3093105-rs3093135构成的单倍型在对照组、IS组及不同性别组中的多态分布。结果CYP4F2基因rs2108622位点GG基因型可能是男性缺血性脑卒中患者的独立的风险因素（P=0.018，OR=2.53，95％CI1.15～5.56），且其风险性主要来源于G等位基因（P=0.013,OR=1.48，95％CI1.09～2.02）。rs2108622-rs3093100．rs3093105-rs3093135构成的GGGT单倍型与男性缺血性脑卒中具有相关性，GGGT单倍型可能是Is的危险单倍型（OR=1.545，95％CI1.144～2.087，P=0.004）。结论CYP4F2基因的多态与男性IS具有相关性，可显著增加男性IS的患病风险。

Objective To investigate the association of polymorphisms of CYP4F2 （cytochrome P450,family 4,subfamily F, polypeptide 2） gene with ischemic stroke（IS） in Han population in northern China.Methods rs2108 622,rs3093100,rs3093105,rs3093135,rs 1558139 SNP of CYP4F2 gene were genotyped in control group,IS group and different gender subgroups by using PCR-RFLP and MALDI-TOF methods.Results rs2108622 GG genotype in CYP4F2 gene was indicated to be independent risk factor of ischemic stroke in man（P=0.018,OR=2.53,95 %CI 1.15～5.56）,and G allele at this SNP particularly showed a correlation with the risk（P=O.O13,0R=l.48,95%CI 1.09～2.02）.GGGT haplotype of rs2108622-rs3093100-rs3093105-rs3093135 SNPs might be an ＂atrisk＂ haplotype of IS in man（OR=1.545,95%CI 1.144～2.087,P=0.O04）.Conclusion CYP4F2 gene polymorphisms correlate with IS in Chinese males.