Tween 80诱导下米曲霉3.5232产胞内脂肪酶的研究

Purification of Lipases from Aspergillus oryzae 3.5232 Induced by Tween 80

米曲霉3.5232在Tween 80诱导下,相比于葡萄糖作为碳源的培养条件,米曲霉在细胞壁、细胞膜及细胞质中均可以诱导产生分子质量为27 kDa的脂肪酶,而在细胞质中又有分子质量为36 kDa的脂肪酶表达。利用硫酸铵沉淀,DEAE Sepahrose Fast Flow离子色谱交换及Sephadex G-75凝胶色谱的分离方法,从米曲霉细胞质中分离纯化了分子质量为36 kDa的脂肪酶。最终纯化倍数为42.45,产率为0.12%。

The expression of the lipase with 27 kDa molecular weight on cell wall, cell membrane and cytoplasm ofAspergillus oryzae 3.5232 was inducted by Tween 80. Another lipase with 36 kDa molecular weight in cytoplasm was also detected under same conditions. The cytoplasmic lipase was purified to homogeneity by ammonium sulfate precipitaiton and DEAE-Sepharose FF and Sepahdex G-75 column chromatography. Purification fold was 42.5 and the recovery rate was 0.12%. Molecular weight of purified lipase was 36 kDa.