发夫酵母工业化生产虾青素的培养基研究

Industrialized Culture Medium of Phaffia rhodozyma for Astaxanthin Production

为进一步降低培养基成本,促进工业化生产,在前期优化的培养基基础上,本研究尝试直接以价廉易得的玉米粉、玉米淀粉、马铃薯淀粉水解液代替葡萄糖作碳源,进行摇瓶发酵实验和10 L罐分批发酵实验,探讨了发夫酵母产虾青素工业化培养基的制备。研究发现玉米淀粉水解液可代替葡萄糖作碳源进行工业化生产,并确定了淀粉双酶水解的最佳工艺条件为淀粉糊浓度为30%、液化酶添加量为25 U/g淀粉、糖化酶添加量为200 U/g淀粉、糖化时间为4 h。这样可大大降低培养基成本。

In order to further reduce the cost of medium and promote industrialized rhodozyma for astaxanthin synthesis was studied on the base ofpre-optimized culture medium, using cheaper corn flour, corn starch and tomato starch hydrolyzate instead of glucose as carbon source. Shake flask culture and 10 liter fermentor culture were used with the mutant 269 of Phaffia rhodozyma. Results showed that com starch hydrolyzate could instead glucose as carbon source in the industrialized production of astaxanthin. The optimal hydrolyzed conditions of corn starch were as follows： the concentration of com starch paste 30%, the concentration of a-amylase 25 U/g starch, the concentration of saccharifying enzyme 200 U/g starch and the saccharifying time 4 hours, under which the cost of culture media could be decrease significantly.