非霍奇金淋巴瘤患者外周血中CD4^+CD25^+调节性T细胞亚群变化初探

The Exploration of Changes of CD4^+CD25^+ Regulatory T Cells in Peripheral Blood of Patients with Non-Hodgkin's Lymphoma

目的:检测非霍奇金淋巴瘤(non-Hodgkin's lymphoma,NHL)患者外周血中CD4+CD25+调节性T细胞(CD4+CD25+regulatoryT cell,Treg)的改变,探讨Treg与NHL的相关性。方法:病例组(n=60)为本院收治的初诊NHL患者,对照组(n=60)为本院健康体检者,用流式细胞技术联合标记CD4、CD25检测对照组及病例组化疗前、化疗后的外周血中CD4+CD25+调节性T细胞的分布特点。结果:(1)病例组化疗前外周血中CD4+细胞比例显著低于对照组(P<0.05),CD4+CD25+调节性T细胞比例显著高于对照组(P<0.05);(2)病例组化疗后,CD4+细胞比例明显高于化疗前(P<0.05),CD4+CD25+调节性T细胞比例明显低于化疗前(P<0.05);(3)病例组化疗后CD4+细胞比例与对照组无显著差异(P>0.05),而CD4+CD25+调节性T细胞比例显著高于对照组(P<0.05)。结论:非霍奇金淋巴瘤患者外周血中CD4+CD25+调节性T细胞比例升高,存在机体免疫抑制,化疗可降低CD4+CD25+调节性T细胞比例。

Objective： To detect the proportion of CD4＋CD25＋ regulatory T cells （Treg） in peripheral blood of patients with non-Hodgkin＇s lymphoma（NHL）, and to explore the relationship between Treg and NHL. Methods： By using flow cytometry with surface staining fluorochrome-conjugated antibodies for CD4, CD25, the percentages of CD4＋CD25＋Treg in peripheral blood of 60 patients with NHL and 60 Healthy controls were detected and analyzed. Results： ①The average peripheral blood CD4＋ levels of patients with NHL before chemotherapy was significant lower than that of the control group （P〈0.05）, while the percentages of CD4＋CD25＋Treg was significant higher than that of the control group （P〈0.05）. ②After chemotherapy, the percentages of CD4＋CD25＋Treg was decreased and CD4＋ levels was increased than before chemotherapy. The difference between them was statistically significant （P〈0.05）.③There was no significant difference between the control group and patients with NHL after chemotherapy of peripheral blood CD4＋ level （P 〉0.05）. But the percentages of CD4＋CD25＋Treg of patients with NHL after chemotherapy was still higher than the control group（P〈0.05）. Conclusion： The increase in Tregs in patients with NHL may induce immune suppression and chemotherapy may cause the decreasing of Tregs.