摘要
目的:检测非霍奇金淋巴瘤(non-Hodgkin's lymphoma,NHL)患者外周血中CD4+CD25+调节性T细胞(CD4+CD25+regulatoryT cell,Treg)的改变,探讨Treg与NHL的相关性。方法:病例组(n=60)为本院收治的初诊NHL患者,对照组(n=60)为本院健康体检者,用流式细胞技术联合标记CD4、CD25检测对照组及病例组化疗前、化疗后的外周血中CD4+CD25+调节性T细胞的分布特点。结果:(1)病例组化疗前外周血中CD4+细胞比例显著低于对照组(P<0.05),CD4+CD25+调节性T细胞比例显著高于对照组(P<0.05);(2)病例组化疗后,CD4+细胞比例明显高于化疗前(P<0.05),CD4+CD25+调节性T细胞比例明显低于化疗前(P<0.05);(3)病例组化疗后CD4+细胞比例与对照组无显著差异(P>0.05),而CD4+CD25+调节性T细胞比例显著高于对照组(P<0.05)。结论:非霍奇金淋巴瘤患者外周血中CD4+CD25+调节性T细胞比例升高,存在机体免疫抑制,化疗可降低CD4+CD25+调节性T细胞比例。
Objective: To detect the proportion of CD4+CD25+ regulatory T cells (Treg) in peripheral blood of patients with non-Hodgkin's lymphoma(NHL), and to explore the relationship between Treg and NHL. Methods: By using flow cytometry with surface staining fluorochrome-conjugated antibodies for CD4, CD25, the percentages of CD4+CD25+Treg in peripheral blood of 60 patients with NHL and 60 Healthy controls were detected and analyzed. Results: ①The average peripheral blood CD4+ levels of patients with NHL before chemotherapy was significant lower than that of the control group (P〈0.05), while the percentages of CD4+CD25+Treg was significant higher than that of the control group (P〈0.05). ②After chemotherapy, the percentages of CD4+CD25+Treg was decreased and CD4+ levels was increased than before chemotherapy. The difference between them was statistically significant (P〈0.05).③There was no significant difference between the control group and patients with NHL after chemotherapy of peripheral blood CD4+ level (P 〉0.05). But the percentages of CD4+CD25+Treg of patients with NHL after chemotherapy was still higher than the control group(P〈0.05). Conclusion: The increase in Tregs in patients with NHL may induce immune suppression and chemotherapy may cause the decreasing of Tregs.
出处
《现代生物医学进展》
CAS
2012年第23期4479-4481,4525,共4页
Progress in Modern Biomedicine