摘要
在过去的20年中,噬菌体抗体库筛选技术被广泛的应用于抗体筛选、疾病治疗,临床诊断以及基础研究之中。在该技术的应用过程中,快速有效的筛选出最适合的单克隆抗体并进行可靠和高效的数据管理和分析是十分重要的。文中总结了近年来噬菌体抗体库的高通量筛选方法并且对数据管理做了简要介绍。
For almost 20 years, phage display antibody （Ab） libraries screening have been widely used in antibody screen, clinical diagnostic, treatment of disease and basic research areas. Rapid identification of the autofit monoclonal antibody, and reliable and efficient data management and analysis are very important in the application of this technology. This review described reported methods for high-throughput screening of antibody phage-display libraries and further more gave a brief introduction to data management.
出处
《现代生物医学进展》
CAS
2012年第23期4577-4583,共7页
Progress in Modern Biomedicine
关键词
噬菌体展示
抗体库
筛选技术
数据管理
Phage display
Antibody library
Screening technology
Data management
