一种随机探针人DNA指纹分析法的建立

A NEW ANONYMOUS PROBE FOR HUMAN DNA FINGERPRINTING

根据随机探针(Anonymous Probe)检测DNA限制性片段长度多态性(RFLPs)分析原理和人与鼠的髓鞘碱性蛋白(MBP)基因cDNA有90％以上同源序列的事实,选用rMBP-cDNA 3′-端非表达区高度重复序列的0.81千碱基对(kb)片段作探针,检测用HaeⅢ重酶解的人DNA限制性片段,结果可以分辨出22条谱带,受检的30例无血缘关系的个体之间,没有两个人的谱带是完全相同的,显示出此方法的高度特异性。本文还比较了若干DNA片段作探针和几种限制性内切酶水解人DNA后检测DNA指纹的初步结果。

Basing on the principle of detecting restriction fragment length polymorphisms (RFLPs) with anonymous DNA probes and the fact that there are more than 90% hom-ologeous sequences in the myelin basic protein (MBP) gene between man and rat, we used the 0.81 kb fragment in highly repeat sequence of the 3'-end non-coding region of rMBP -cDNA as a probe to detect the RFLPs of human DNA-digested with HaeⅢ, and 22 bands could be found on the autoradiograph.es. It was not found the identical autoradio-graphes between two unrelated persons tested. It shows that this method is highly indivi-dualspecific. Meanwhile this paper compares the results of DNA fingerprinting by several DNA probes and restriction endonucleases.