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Anti-NEP1-40多克隆抗体的制备与检测

Preparation and detect of NEP1-40 polyclonal antibodies
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摘要 目的制备Anti-NEP1-40多克隆抗体。方法合成NEP1-40多肽,采用弗氏佐剂制备免疫抗原,多次多点皮下注射健康雄性家兔,刺激机体产生相应抗体,颈动脉放血分离兔血清。结果获得含NEP1-40抗体的免疫血清,其抗体特异性好,亲和力较高。结论 NEP1-40兔多克隆抗体可以满足NEP1-40相关的科学研究。 Objective To prepare NEP1-40 polyclonal antibodies.Methods NEP1-40 antigen was subcutaneously injected into health male rabbits.The blood was collected from carotid artery.Results NEP1-40 antibody was got from the serum with affinity and specialty.Conclusion Subcutaneous immune method can get NEP1-40 antibody.
出处 《西部医学》 2012年第8期1439-1440,1445,共3页 Medical Journal of West China
基金 国家自然科学基金(编号:81071060) 西安医学院大学生科研项目(编号:10DXS06-07) 西安医学院质量工程检验实验示范中心(编号:XYZL2010-18)
关键词 NEP1-40 多克隆抗体 神经再生 NEP1-40 antigen Polyclonal antibody Nerve regeneration
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