姜黄素对孤独症大鼠行为及脑源性神经营养因子表达的影响

Effect of Curcumin on Behavior of Autism Rats and the Expression of Brain Derived Neurotrophic Factor

目的研究姜黄素对孤独症鼠行为及脑源性神经营养因子(BDNF)表达的影响。方法参照Schneider的方法在大鼠孕12.5 d时模型组母鼠腹腔注射丙戊酸钠(VPA)600 mg.kg-1建立孤独症动物模型,对仔鼠进行发育学、行为学、形态学鉴定。随机选取35 d孤独症鼠20只、盐水(NS)组鼠10只。NS组鼠为出正常对照组,孤独症鼠随机分为孤独症溶剂对照(VC)组10只、孤独症姜黄素干预(VPA-C)组10只。VPA-C组鼠于出生后35 d连续2周腹腔注射姜黄素50 mg.kg-1[姜黄素用含1 mL.L-1二甲基亚砜(DMSO)的PBS液配成10 g.L-1的溶液]。NS组、VC组鼠于生后35 d连续2周每日腹腔注射等量的含1 mL.L-1DMSO的PBS液。观察姜黄素对孤独症鼠社交行为及重复理毛行为的影响,应用免疫组织化学染色法观察姜黄素干预后BDNF的表达情况。应用SPSS 18.0软件进行统计学处理。结果姜黄素干预后,孤独症鼠社交行为较干预前次数增加(P<0.05),社交行为潜伏期缩短(P<0.001),重复理毛行为累积时间减少(P<0.01);姜黄素干预后孤独症鼠大脑颞叶皮质BDNF阳性细胞积分光密度(IOD)值较干预前增加(P<0.05)。结论姜黄素对孤独症有一定的治疗作用,可一定程度改善孤独症鼠异常行为,其机制可能通过增加BDNF表达发挥作用。

Objective To study the effect of curcumin on behavior of autism rats and expression of brain derived neurotrophic factor （BDNF）. Methods Animal model of autism was established according to Schneider method,the female that received a single intraperitoneal injection of 600 g·L-1 sodium valproate on the 12.5 day after conception and then the auxanology, behavioral and morphological test were performed to identify autism mouse. Randomly selected animal model of autism （n = 20） and offspring of the NS group （n = 10）at age of 35 days , the saline group rats were designated as normal control （Con）group, the animal model of autism was randomly divided into vehiclecontrol group （ VC group, n = 10 ） and curcumin group （ VPA - C group, n = 10 ）, the VPA - C group was injected curcumiu into abdominal for 2 weeks（ concentration of curcumin was 10 g·L-1 which was dissolved in PBS containing 1 mL · L^-1 dimethyl sulfoxide）. The solvent control group and normal control group were given PBS with volume of 1 mL · L^-1 DMSO for 2 weeks. This investigation was observed the effects of curcumin on rat behavior and used the immunohistocbemical method to observe the expression of BDNF in the different groups . The results were statistically assessed by SPSS 18.0 software. Results The effects of curcumin on autism rats were starting to increase the number of social behavior （P 〈 0.05 ）, to decrease latency to social behavior （P 〈 0. 001 ） , and to reduce repeat behavior of autism mouse （P 〈 0.01 ） ; Curcumin increased BDNF integral optical density values of autism rats in temporal cortex （ P 〈 0.05 ）. Conclusions Curcumin have a therapeutic effect on rats with autism,it can be improved abnormal behavior of autism rats to some extent, the mechanism may be to play a role by increasing the expression of BDNF.