中国(广州地区)正常人群EAP表型及血痕EAP表型的检测

THE DETERMINATION OF EAP PHENOTYPES OF CHINESE POPULTAION IN GUANGZHOU AREA AND EAP PHENOTYPING OF BLOODSTAINS

本文介绍用琼脂糖凝胶电泳法对广州地区523例健康无关个体EAP表型的检测结果。红细胞酸性磷酸酶(erythrocyte acid phosphatase简称EAP)A型32例(6.1％),EAP BA型175例(33.5％);EAP B型316例(60.4％),此外还发现了5例变异型EAP RA。同时还检测了58例ESR升高患者的EAP表型,其结果与正常人群比较无显著性差异(P>0.05)。对血痕EAP表型检测的结果表明,在室温下保存5周的血痕EAP表型能全部正确定型,有少数保存6个月仍能正确定型。我们认为,用此方法检测EAP表型有酶谱带清晰、方法简便,试剂价廉且易得到等优点,值得推广。

The results of EAP phenotyping of Chinese population(N = 523) in Guangzhou area, using the agarose gel electrophoresis, were presented. Basing on the distribution of EAP phenotypes in this population, EAP A = 32(6.1%), EAP BA =175(33.5%), and EAP B = 316(60.4%), the gene frequencies of EAP3 and EAPb were calculated as 0.2285 and 0.7715. respectively, it was consistent with the Hardy-Weinberg's equilibrium. We found the variant EAP RA in 5 cases. Meanwhile, the EAP phenotypes of 53 patients, whose ESR were over the normal level, were determined. Comparing the results of EAP phenotyping of patient-group with that of healthy group, no significant difference existed. The results of determination of EAP phenotypes in bloodstains showed that EAP phenotypes could be detected correctly in bloodstains stored at room temperature within 5 weeks.