葡萄糖-6-磷酸脱氢酶缺乏致新生儿黄疸溶血机理探讨——Ⅰ.抗氧化红细胞酶活性分析

STUDIES ON THE MECHANISM OF G6PD DEFICIENCY RELATED NEONATAL JAUNDICE Ⅰ: ANANALYSIS OF ERYTHROCYTE ANTIOXIDATIVE ENZYMES ACTIVITIES

对红细胞谷胱甘肽过氧化酶(GSH-Px),谷胱甘肽还原酶(GR)、超氧化物歧化酶(SOD)及谷胱甘肽(GSH)含量进行了测定,结果显示:①新生儿GSH-Px活性显著低于成人,这可能与新生儿抗氧化能力低下有一定关系;②G6PD缺乏的成人GR活性显著高于正常成人,提示GR活性增高在一定程度上能代偿G6PD活性和GSH含量下降造成的影响;③G6PD缺乏的成人及新生儿GSH含量均显著低于相应正常对照,但GSH含量降低可能不是导致新生儿溶血的重要环节。

In order to explore the mechanism of glucose-6-phosphate dehydrogenase (G6PD) defi-cency related neonatal Jaundice, three erythrocyte enzymes and reduced glutathione(GSH) contents were studied. The results showed that (1) neonatal erythrocyte glutathione per-oxidase(GSH-Px) activity was significantly lower than adult's, (2) glutathione reductase (GR) activity in G6PD deficient adults was significantly higher than those in normal adults, (3) decrease in erythrocyte GSH were found in G6pD deficient adults and new-borns. The results suggested that decreased GSH-Px activity in neonatal erythrocytes might interfere antioxidative activity, the low level of GSH in G6PD deficient newborns seems not an important factor in causing neonatal jaundice. Increased GR avtivity in G6PD deficient adults may compensate diminished G6PD activity and GSH in erythrocytes.