常温与低温灌注下狗全肝血流阻断比较实验

COMPARATIVE EXPERIMENTAL STUDY OF THE CANINE HEPATIC VASCULAR EXCLUSION UNDER THE NORMOTHERMIC CONDITION AND HYPOTHERMIC PERFUSION

为了探讨全肝血流阻断带来的生理生化改变,常温下全肝血流阻断的安全时限及低温灌注下对肝脏的保护作用,作者采用脾颈静脉分流减压门静脉系统方法,将21只狗随机分为3组:A组全肝血流阻断45分钟;B组1小时;C组在低温灌注下全肝血流阻断1小时,观察动物一般情况,生理生化及病理学改变和术后生存率。结果表明,常温下狗全肝血流阻断安全时限为45分钟;超过1小时,动物发生休克,生理、生化、病理改变显著,术后存活率仅28.5％;低温灌注可起到明显的对肝脏缺血的保护作用。

In order to find out the biochemical and physiological changes of total hepatic vascular exclusion(THVE), the critical length of the hepatic ischemic period of the normother-mic THVE and the protective effects of the hypothermic perfusion on the hepatic ischemia, the authors randomized 21 dogs into 3 groups: group A, THVE for 45 minutes; group B, THVE for 60 minutes; group C, THVE with hypothermic perfusion for 60 minutes. The postoperative course, survival rates, biochemical, hemodynamic metabolic consequences and pathological changes of the animals were observed. It has been shown that the tolerance of the canine liver for THVE appeares to be 45 minutes, the critical period of 60 minutes of THVE is followed by an increasing number of death (surival rate of group B: 285.%) and severe metabolic upsets and the hypothermic perfuoisn has obvious protective effects on the liver ischemia.