原发型肝癌的HBV感染及肝内的HBVDNA

INFECTION OF HEPATITIS B VIRUS IN HEPATOCELLULAR CARCINOMA: AN ANALYSIS OF HBV DNA IN SERUM AND LIVER

应用^(32)P标记的3.2kb克隆HBVDNA探针对36例原发型肝癌患者的血清和肝组织作HBVDNA斑点杂交,结果:血清、肝癌组织、癌近邻肝组织和癌远邻肝组织的阳性率分别为56％、91％88％和80％,皆高于国内外一些同类研究,说明其HBV感染率比一般认为的还要常见,尽管肝内良恶组织的HBV DNA阳性率未见显著差别。此外,尚对HBV6项血清标记作了检测,结果HBsAg和PHSA·Re的阳性率均较高,分别为83％和75％,说明多数病例的HBV感染仍然处于病毒活动复制阶段。由于血清HBsAb阳性者,肝内HBV DNA全阳性,提示HCC阶段的“保护性抗体”并不能清除病毒感染。

To investigate the hepatitis B virus (HBV) infection in hepatocellular carcinomas (HCCs), 36 patients with HCC were studied for HBV DNA in their sera and livers. By dot-blot hybridization with 32P-probe of 3.2kb, HBV DNA was pick up from 20/36 (56%) of sera, 29/32 (91%) of cancerous, 29/33 (88%) of peri-canc,erous and 16/20 (80%) of non-cancerous tissues. All the positivities were higher than that of some other reports when compared showing the closer correlation between HBV infection and HCC development in southern China. On the other hand, 6 serological HBV markers were also detected with respective positivities of HBsAg and PHSA.Re to be 83% (30/36) and 75% (27/ 36) suggesting the highly active replication of HBV in the majority of HCCs. Since HBsAb positive patients all had HBV DNA in their livers, the inability of this 'protective' antibody to eliminate HBV infection was implied when HCC had developed.