人大肠肥大细胞多态性光镜特征(两种新类群:CTMC-1与MMC-1的发现)

MORPHOLOGICAL FEATURES FOR DIFFERENT POPULATION OF MAST CELL IN HUMAN LARGE BOWEL: A DISCOVERY OF TWO NEW GROUPS, CTMC-1 AND MMC-1

人体肥大细胞的多态性已受到广泛关注,对于肥大细胞分型的研究已成为这一问题的关键。本实验研究了42例人大肠4种肥大细胞类群光镜特征,其中两种为作者的新近发现,暂命名为结缔组织肥大细胞-1(CTMC-1)和粘膜肥大细胞-1(MMC-1)。

Great attention has paid on the polymorphisms of human mast cells, that the classification of these cells has become the key point. In this study, the microscopic features of 4 populations of mast cells from 42 specimens of human large bowel were carried out. The tissue sections were made and stained with toluidine blue, Alcian blue- safranin, neutral red or Wright-Giemsa mixtures. The critical electrolyte concentration (CEC) method was performed to predetermine the CEC values for different population of mast cells. Based on the staining properties of cytoplasmic granules, morphological characteristics and distribution of these cells, two new population, connective tissue mast cell-1 (CTMC-1) and mucosal mast cell-1 (MMC-1) were discovered in some specimen in addition to those two population, CTMC and MMC that were similar to rodent mast cells and found normally in tissues. CTMC-1 and MMC-1 were large cells containing coarse cytoplasmic granules. Some of their histochemical properties were also reported. This discovery would benefit further study of the heterogeneity of human mast cells.