骆驼蓬碱、氟尿嘧啶对视网膜母细胞瘤细胞系SO-Rb50生物学效应

EFFECTS OF HARMALINE AND FLUOROURACILUM ON HUMAN RETINOBLASTOMA CELL LINE SO-RB50

在体外检测了骆驼蓬总碱,氟尿嘧啶(fluorouracilum,5-Fu)对人视网膜母细胞瘤细胞系SO-Rb50的生物学效应。两种药物对该细胞的半数抑制浓度(IC_(50))分别是1.437μg/ml与5.321μg/ml,提示Rb细胞在体外对化疗敏感。临床Rb化疗效果欠佳可能与血-视网膜屏障有关。

Harmaline and Fluorouracilum(5-Fu) were incubated in vitro together with human retinoblastoma(Rb) cell line SO-Rb50 for 72 hours, then subjected to sampling, viable cell counting and inhibition rate(IR) calculating. When concentration in12μg/ml, the IR of Harmaline to SO-Rbso cells was 96.6±1.98% and 5-Fu to SO-Rb50 cells was 93.5 ±0.9%. the 50% inhibition concentration (IC50) was 1.437μg/ml in Harmaline test and 0.32μlg/ml in 5-Fu test. Rb cells were sensitive to chemical therapy in vitro, but not in the clinical therapy, the blood-retina barrier might be the responsible factor.