涎腺肿瘤免疫组化研究

AN IMMUNOHISTOCHEMICAL STUDY OF THE SALIVARY GLAND NEOPLASM

应用6种抗体(CEA、MAK-6、EMA、S-100、GFAP、HHF-35)对55例涎腺肿瘤和9例肿瘤旁涎腺组织进行免疫组化研究,结果表明:肿瘤旁涎腺组织的免疫光谱,以腺管上皮细胞为一端,肌上皮细胞为另一端,润管细胞和腺泡细胞位于光谱中间段,多形性腺瘤起源于同一类细胞,而不是混合性起源。简略介绍HHF-35的性质和应用价值,在识别肌上皮细胞方面,HHF-35比GFAP更为敏感。

Fifty-five cases of salivary gland neoplasm and 9 cases of salivary gland para-tumor (SGPT) were studied with six immunologic antibodies (including; carcinoembryonic antigen-CEA, monoclonal antibody keratin(MAK)-6, epithelial membrane antigen(EMA) S-100 protein-S-100, glial fibrillary acidic protein(GFAP) and muscle-actin-specific monoclonal antibody(HHF35) by immunohistochemical methods. Experimental results demonstrated the duct cells situated at the end of the immunologic spectrum of SGPT, myo-epithelia at the other end. Intercalated duct cells and acinar cells occupied a medial portion of the spectrum. On the other hand, in the pleomorphic adenoma, duct cells were widespread possitive with some epithelial marker antibodies (such as, CEA, MAK-6, EMA). Stellate cells of the myxoid region and chondroid cells presented immunoreaction with S-100, GFAP, HHF35. Cells of the solid epithelial areas coexpressed positive reaction with both epithelial and non-epithelial antibodies as markers. So, the pleomorphic adenoma of salivary gland was thought to arise from a single origin rather than a mixed origin of tumor. In order to identify myoepithelium, obviously, HHF35 is more sensitive than GFAP.