一株假丝酵母合成GSH的发酵条件优化

Research on fermentation condition for glutathione with Candida

通过对1株假丝酵母诱变株发酵合成谷胱甘肽的条件进行研究,发现最佳培养基为葡萄糖4%,蛋白胨1.7%,磷酸二氢钾0.2%,硫酸铵0.6%,硫酸镁0.14%,硫酸锌0.001%,控制发酵条件初始pH值5.0,发酵温度25℃,装液量40mL/250mL,发酵时间66h,发酵后谷胱甘肽的产量达到了200.62mg/L。

The candida of mutntion fermentation condition of GSH was studied,the result showed： the optimum medium is glucose 4%,peptone 1.7%,KH2PO4 0.2%,（NH4）2SO4 0.6%,MgSO4 0.14%,ZnSO4 0.001%,the optimum condition were initial pH value 5.0,temperature 25℃,volume 40ml/250ml.It was fermented for 66h,the final production of GSH was 200.62mg/L.