摘要
通过诱变获得高产胞外虫草素和虫草多糖的蛹虫草菌株。采用紫外线诱变(UV)、化学诱变(LiCl)、复合诱变(UV-LiCl)3种方式对蛹虫草孢子进行诱变;发酵检测存活菌株的胞外虫草素和虫草多糖的含量。结果:以胞外虫草素为指标,3种诱变方式的最大正突变率分别为化学突变(29.2%)>紫外突变(28.6%)>复合诱变(26.5%);以胞外多糖为指标,最大正突变率分别为紫外诱变(35.7%)>复合诱变(33.3%)>化学诱变(27.0%)。紫外诱变突变株Z-5-1胞外虫草素产量达0.842g/L,比出发菌株高311%;紫外诱变突变株Z-4-7胞外虫草多糖产量达5.250g/L,比出发菌株高148%。在连续培养5代后,仍具有较好的遗传稳定性。紫外诱变能获得较高的蛹虫草正突变率,同时能获得高产虫草素、虫草多糖的突变株。
The aim was to obtain high-yield strains of extracellular cordycepin and polysaccharide of Cordyceps militaris.Three kind of mutation way,ultraviolet radiation(UV)、lithium chloride(LiCl) and compound mutagenesis(UV-LiCl) were used to deal with the spores of Cordyceps militaris.Fermenting the survival strains and detecting their content of extracellular cordycepin and cordyceps polysaccharide.The results showed: the maximum positive mutation rate order of extracellular cordycepin was LiCl(29.2%) 〉 UV(28.6%) 〉 UV-LiCl(26.5%) respectively,and that of extracellular polysaccharide was UV(35.7%) 〉 UV-LiCl(33.3%) 〉 LiCl(27.0%).The UV-induced mutant strain Z-5-1 had the highest extracellular cordycepin yield of 0.842g/L,which was 311% higher than that of the original strain.And the UV-induced mutant strain Z-4-7 had the highest extracellular Cordyceps polysaccharide production of 5.250g/L,which was 148% higher than that of the original strain.UV mutagenesis could obtain a higher positive mutation rate for Cordyceps militaris,while could produce good mutant strains with high-yield of extracellular cordycepin and extracellular cordyceps polysaccharide.
出处
《中国酿造》
CAS
2012年第7期57-61,共5页
China Brewing
基金
贵州省教育厅自然科学研究项目(黔教科(2009)0129号)
贵州省"十二五"农业科技攻关项目(黔科合NY字[2011]3054号)
贵州大学自然科学青年科研基金(N0:(2007)076)
关键词
蛹虫草
胞外
虫草素
虫草多糖
诱变
Cordyceps militaris; extracellular; cordycepin; polysaccharide; mutagenesis