GC-MS定量检测谷氨酸发酵产酸期细胞膜脂肪酸

Quantitative determination of fatty acids in cell membrane of Corynebacterium glutamicum during glutamic acid production by GC-MS

采用气相色谱质谱连用法(GC-MS)分析了谷氨酸棒状杆菌的细胞膜中脂肪酸的组分,并用选择离子检测方式(SIM)测定产酸期前后细胞膜脂肪酸含量的变化。结果表明:谷氨酸棒状杆菌细胞膜主要含有肉豆蔻酸、棕榈油酸、棕榈酸、硬脂酸和油酸等5种脂肪酸,其中棕榈酸含量最高,棕榈油酸含量最低;另外,在谷氨酸发酵过程中,随着产酸的启动,细胞膜中饱和脂肪酸与不饱和脂肪酸比率呈下降趋势,说明细胞膜中饱和脂肪酸与不饱和脂肪酸的比率与谷氨酸的分泌可能存在一定的关联性。

The fatty acids in cell membrane of Corynebacterium glutamicum during glutamic acid production were quantitatively determined by GC-MS（SIM）.The results showed that myristic acid,palmitoleic acid,palmitic acid,oleic acid and stearic acid were the main fatty acids in cell membrane of C.glutamicum.Among the 5 main fatty acids,the content of palmitic acid was highest,whereas the content of palmitoleic acid was lowest.Additionally,the ratio of saturated fatty acids to unsaturated fatty acids decreased after the biosyhthesis of glutamic acid initiated.It indicated that there might be a close relationship between the secretion of glutamic acid and the ratio of saturated fatty acids to unsaturated ones.