宁心通痹胶囊对大鼠微循环、血液流变学及凝血功能的影响

Effect of Ninxintongbi capsule on microcirculation,hemorheology and coagulation function in rats

目的:研究宁心通痹胶囊对大鼠微循环、血液流变学及凝血功能的影响。方法:(1)小鼠注射去甲肾上腺素(Adr),观察给予Adr前后8 min小鼠耳廓微循环情况。(2)大鼠给药7 d,以高分子右旋糖酐建立大鼠血淤证模型,测定血液流变学各项指标。(3)大鼠给药28 d,测定凝血功能各项指标。结果:(1)宁心通痹胶囊中、高剂量组与模型对照组比较,A、V血管收缩率和毛细血管网交叉点减少率明显降低(P<0.01,P<0.05)。(2)宁心通痹胶囊能显著降低小鼠全血黏度和红细胞压积,与模型对照组比较有显著性差异(P<0.05,P<0.01)。(3)宁心通痹胶囊低、高剂量组均能降低大鼠PLT聚集率和延长大鼠的TT,与模型对照组比较有显著性差异(P<0.05).结论:宁心通痹胶囊有改善微循环,降低血液黏度,减少血栓形成的作用。

OBJECTIVE To observe the effects of Ninxintongbi capsule on microcirculation,hemorheology and coagulation function in rats.METHODS（1）The mice were given Adr,the auricle microcirculation of mice was observed before and 8 minutes after drug administration.（2）The rats were fed with Ninxintongbi capsule,after 7 days,the blood-stasis model rats were made by injection of high molecule dextran,then observed the hemorheology index.（3）The rats were fed with Ninxintongbi capsule,after 28 days,then observed the coagulation function.RESULTS（1）Compared with the model group,high and medium doses of Ninxintongbi capsule could decrease the contract ratio of the diameter of artery and vein,reduce ratio of micrangium crossing net（P〈0.01,P〈0.05）.（2）Compared with the model group,Ninxintongbi capsule could reduce blood viscosity and hematocrit（HCT）（P〈0.05,P〈0.01）.（3）Compared with the model group,Ninxintongbi capsule could reduce platelet aggregation and prolong plasma thrombin time（TT） P〈0.05.CONCLUSION The results showed that Ninxintongbi capsule have effects of improving auricle microcirculation,decreasing blood viscosity and thrombosis.