养精胶囊对老年SD大鼠性功能的影响

Effects of Yangjing capsules on sexual function of SD rats

目的观察养精胶囊对老年雄性SD大鼠性功能的影响，并探讨其可能的影响机制。方法将40只老年雄性SD大鼠随机分为实验组和对照组，实验组给予养精胶囊（0．5g／kg·d^-1）灌胃4周，对照组给予等剂量生理盐水灌胃相同时间。4周后通过观察两组大鼠阴茎勃起变化、检测血清睾酮水平和免疫组化方法检测大鼠阴茎海绵体组织中内皮型一氧化氮合酶（eNOS）及海绵体组织血管中CD34分子表达的变化情况等评价大鼠性功能变化情况。结果（1）对照组老年大鼠干预后血清总睾酮水平较干预前下降，但组间比较差异无统计学意义（P〉0．05）；养精胶囊组老年大鼠干预后血清总睾酮水平较干预前有所提高，但差异亦无统计学意义（P〉0．05）；（2）在老年大鼠性功能指标方面：养精胶囊干预组老年大鼠阴茎勃起次数、勃起潜伏期及勃起维持时间均较对照组有所改善。（3）阴茎海绵体组织HE染色结果提示：养精胶囊组老年大鼠阴茎海绵体组织血管管腔明显扩张，充血，管腔内壁变薄；对照组老年大鼠阴茎海绵体组织内，血管管腔未见明显充盈扩张，管腔内充血不明显，且管腔有大量残留的陈旧性血液。（4）免疫组化结果提示：两组大鼠海绵体组织中，均可见深染的棕褐色阳性颗粒，但深染程度和颗粒分布密度不一。低倍镜（×100）视野下观察，药物干预后实验组大鼠阴茎海绵体组织中eNOS的阳性颗粒的数量明显高于对照组（P〈0．05）；（5）微血管密度结果提示：实验组老年大鼠阴茎海绵体组织血管内皮中CD34的表达明显强于对照组，高倍镜下（×400）观察，实验组微血管密度MVD值较对照组升高（P〈0．05）。结论养精胶囊能促进血清睾酮的分泌，增强阴茎海绵体组织内eNOS的表达，这可能是养精胶囊改善大鼠性功能和阴茎勃起的作用机制。

Objective To investigate the effects of Yangjing Capsules on the erectile function of rats and explore its mechanism. Methods Forty rats were divided into two groups randomly, i.e. the experimental group and the control group. The rats in the experimental group were fed with Yangjing Capsules at 0.5g/kg . d-1 per day for 4 weeks, and those in the control group were fed with the normal saline at the same dosage and in the same duration. All the rats were killed on the 4th week, and then the level of testosterone, the expression of nitric oxide synthase and CD34 in the corpus cavernosum penis were detected. Results 1. In the control group, the level of total serum testosterone of the adult rats after intervention was lower than those before intervention, but no significant difference was found between the two groups （P〉0.05）; In the first group, the level of total serum testosterone was elevated after intervention, but no significant difference was found（P〉0.05）; 2. The penile erection, erectile latent time and maintenance of erection of rats in the experimental group were all improved significantly, compared with that of the control group; 3. The HE staining analysis of penile corpus cavernosum tissues of the adult rats in the experimental group showed that the vascular lumen was dilated, congested remarkably, and the inner wall got thinner, but there were no obvious changes in the control group, and in the lumens there was much residual old blood; 4. The immunohistochemical analysis showed lots ofpositive deeply dyed brown granules in the rat corpus cavernosum tissues of the two groups, but they differed in the degree of staining and particle density. After intervention the expression of eNOS in the rat corpus cavernosum tissues in the experimental group was significantly higher than that in the control group（P〈0.05）; 5. The expression of CD34 in the cavernous vascular endothelial tissues of the rats in the experimental group was higher than that of the control group, and the average MVD of the experimental group increased compared with that of the control group （P〈0.05）. Conclusion Yangiing Capsules might improve the sexual function of the adult rats by upregulation of eNOS in the corpus cavernosum of penis and promotion of the secretion of serum testosterone.