黔产乌药化学成分研究

Chemical Constituents of Lindera aggregata

目的:研究樟科山胡椒属植物乌药块根Lindera aggregata的化学成分和抗氧化活性。方法:采用硅胶柱色谱、Sephadex LH-20色谱、D-101大孔树脂色谱等方法进行分离、纯化,通过光谱分析法鉴定它们的结构并对化合物进行抗氧化活性测试。结果:分离得到12个化合物,分别为hernangerine(1),syringgaresinol(2),香草醛(vanilin,3),α-cadinol(4),对羟基苯甲醛(p-hydroxybenzaldehyde,5),β-谷甾醇亚油酸酯(β-sistosterol linoleate,6),表儿茶素(epicatechin,7),boldine(8),norboldine(9),norboldine acetate(10),胡萝卜苷(daucosterol,11)和β-谷甾醇(β-sitosterol,12)。用DPPH法测定化合物1,2,3,4,7,9,10的抗氧化活性。结论:化合物1～6均为首次从该植物中分离得到,化合物2,7有一定的抗氧化活性。

Objective： To investigate chemical constituents and antioxidant activities of Lindera aggregata. Method： Silica gel chromatography, D-101 macroporous resin and sephadex LH-20 were used for separation. Spectroscopic techniques were used for structure identification of compounds. 1, 1-diphenyl-2-picrylhdrazyl radical （DPPH） method was used for antioxidant activities bioassay of seven compounds. Result ： Twelve compounds were obtained and determined to be hernangerine （1）, syringgaresinol （2）, vanilin （3）, α-cadinol （4）, p- hydroxybenzaldehyde （ 5 ）, β-sistosterol linoleate （ 6 ）, epicatechin （ 7 ）, boldine （ 8 ）, norboldine （ 9 ）, norboldine acetate （10）, daucosterol （11） and/3-sitosterol （12）. The antioxi＇dant activities of compounds 1, 2, 3, 4, 7, 9 and 10 were evaluated through DPPH assay. Conclusion： Compounds 1-6 were isolated from this genus for the first time. Compounds 2 and 7 exhibited moderate antioxidant activities.