小丛红景天脂溶性成分及抗菌活性分析

GC-MS Analysis of Fat-soluble Compositions and Antimicrobial Activity of Rhodiola dumulosa

目的:分析小丛红景天中脂溶性成分组成及体外抗菌抑菌活性。方法:采用索氏提取法,提取陕西太白山产小丛红景天中脂溶性物质,经甲酯化处理后用气相色谱-质谱联用技术(GC-MC)分离和鉴定脂溶性成分的种类及含量。通过体外实验,分析研究小丛红景天脂溶性成分抗菌抑菌活性。结果:脂溶性成分共分离出三个馏分,即:非极性馏分、弱极性馏分和极性馏分。其中非极性馏分成分20种,弱极性馏分成分17种,极性馏分成分12种。抑菌实验表明,小丛红景天脂溶性成分对选定的白假丝酵母菌(CMCC85021)、枯草芽胞杆菌(CMCC63501)、金黄色葡萄球菌(ATCC25925)和大肠埃希氏菌(ATCC25922)四种供试菌株均有很好的抑制作用。结论:小丛红景天中含有一些重要的脂肪酸成分,具有较高的保健营养价值。同时,其脂溶性成分具有很好的抑菌杀菌活性。

Objective： To assay antibacterial activity and fat-soluble compositions of Rhodiola dumulosa. Method： The fat-soluble components of R. dumulosa produced in Taibai mountain in Shanxi province were extracted by Soxhlet extraction. The category and content of fat-soluble components were identified and determined by gas chromatography-mass spectrometry （GC-MS） after methyl esterification treatment. The antibacterial activity of fat-soluble components was assayed in vitro. Result： Total 3 kinds of fraction were identified from the fat-soluble components from R. dumulosa. ： the non-polarity compounds, weak polarity compounds and polarity compounds. Total 20 kinds of compositions were identified from the non-polarity compounds. Total 17 kinds of compositions were identified from the weak-polarity compounds. Total 12 kinds of compositions were identified from the polarity compounds. The result of antibacterial activity of the fat-soluble component in vitro showed that Escherichia coli, Staphylococcus aureus, Bacillus subtillis and Candida albicans have a better antibacterial activity. Conclusion： It was shown that there were some impotent fat-soluble components in R. dumulosa which have potential value in food nutrition and health care. At the same time, the fat-soluble components have better antibacterial activity：