新疆产孜然抗氧化活性研究

Antioxidant Activity of Different Solvent Extracts of Cuminum cyminum from Xinjiang

目的:研究新疆产孜然抗氧化活性。方法:采用,二苯代苦味酰基自由基(DPPH),2,2'连氨-双-(3-乙基苯并噻唑啉-6-磺酸)二胺盐(ABTS)和还原能力法研究了新疆孜然抗氧化活性,并测定孜然中的总酚和总黄酮的含量。结果:孜然提取物具有不同程度的抗氧化能力,甲醇和乙酸乙酯提取物的抗氧化活性相对优于其他溶剂萃取物。不同溶剂萃取物中总酚和总黄酮含量有较明显差异。结论:同种溶剂提取物对不同抗氧化能力存在一定差异,而4种提取物在同一个抗氧化评价指标上也有差距;孜然不同溶剂提取物的抗氧化活性与酚类和黄酮类化合物具有很大相关性。

Objective： To study the antioxidant activity of Cuminum cyminum from Xinjiang. Method： Antioxidant activities of the samples were determined by three different test systems, 1, 1-diphenyl-2- picrylhydrazyl radical （DPPH）, 2, 2＇-azino-bis （3-ethylbenzthiazoline-6-sulfonie acid （ABTS） and reducing power assay and the contents of total phenolics and total flavonoids were also tested. Result： Samples showed different antioxidant ability, the antioxidant of methanol and ethyl acetate extract were significantly stronger than the others; the contents of total phenolic and flavonoids of extracts had obvious difference. Conclusion： The results showed that their antioxidant activities have significant discrepancy, having close relations with extraction solvent and activity assay methods. It is extremely important to point out that; there is a positive correlation between antioxidant activity potential and amount of phenolic and flavonoids compounds of the extracts.