菟丝子外用对实验性豚鼠白癜风的药效

Pharmacological Effect of Cuscutae Semen by External Use on Experimental Vitiligo in Guinea Pigs

目的:考察菟丝子外用对实验性白癜风豚鼠模型的治疗作用,为《中国药典》增补其"外用消风祛斑"的功效提供药效学依据。方法:采用H2O2化学脱色法制备实验性白癜风豚鼠模型,观察菟丝子1.86,0.93,0.49 g.kg-1外涂40 d对豚鼠皮肤黑色素分布及血浆中酪氨酸酶(TYR)、血清胆碱酯酶(CHE)和单胺氧化酶(MAO)活性的影响。结果:菟丝子外用对实验性白癜风有良好的治疗作用,给药组皮肤黑色素分布含量增多,与模型组比较,菟丝子高、中剂量组具有极显著性差异(P<0.01,P<0.05);菟丝子高、中剂量血浆TYR[(30.57±3.39),(24.33±1.89)ng.mL-1]明显高于模型组[(16.43±3.50)ng.mL-1](P<0.01);菟丝子高剂量血清CHE[(94.20±6.73)U.mL-1]明显高于模型组[(86.55±5.61)U.mL-1](P<0.01);菟丝子高剂量血清MAO[(36.42±3.99)U.mL-1]明显低于模型组[(40.59±4.04)U.mL-1](P<0.05)。结论:菟丝子外用对白癜风豚鼠模型有治疗作用。

Objective： To investigate pharmacological effect of Cuscutae Semen by external use on experimental vitiligo in guinea pigs, and to provide the reference basis for the 〈 Chinese Pharmacopeia〉 （ChP）. Method： The vitiligo model was produced by chemical decoloration method. The Cuscutae Semen preparation was externally applied to the guinea pig skin （ 1.86, 0.93, 0.49 g ·kg ^-1 ） for 40 days. Melanin distribution in skin and tyrosinase （TYR） , cholinesterase （CHE） and monoamine oxidase （MAO） in blood were determined. Result ： The Cuscutae Semen showed good effects. Compared with the model group, it increased the skin melanin generation. Compared with the models, the Cuscutae Semen of 1.86 g·kg^-1 groups had significant difference （P 〈0.01 ） , the Cuscutae Semen of 0.93 g·kg^-1 group also showed difference （P 〈 0.05）. For TYR, compared with the models （16.43±3.50） ng ·mL^-1, 1.86 g ·kg^-1 and 0.93 g·kg^-1 groups of Cuscutae Semen showed significantly difference （30.57 ±3.39） ng ·mL^-land （24.33± 1.89） ng·mL^-1 respectively （P 〈0.01 ）. Compared with the models, the Cuscutae Semen of 1.86 g ·kg^-1 group increased the activity of CHE [ （94.20±6.73） U·mL^-1], （P 〈 0.01 ） and reduced the activity of MAO [ （36.42± 3.99） U·mL^- 1 （p 〈 0.05 ）. Conclusion ： The Cuscutae Semen by external use shows good effects on vitiligo in guinea pigs.