五子衍宗丸对大鼠交配功能和肾阳虚模型小鼠的影响

Experimental Study of Wuzi Yanzong Wan on Mating Capacity of Rats and the Kidney-yang Deficiency Mice

目的:研究五子衍宗丸对大鼠交配功能及肾阳虚小鼠的影响,探讨该药补肾壮阳的药理作用。方法:①选用Wistar种系雄性大鼠,随机分为正常对照组、男宝组(0.35 g.kg-1)、五子衍宗丸高、中、低剂量组(2.0,1.0,0.5 g.kg-1),ig给药28 d,与摘除卵巢14 d的雌鼠合笼观察其交配能力。测定雄鼠扑捉潜伏期、射精潜伏期、扑捉次数及射精次数。取血测定血清睾酮(T)、黄体生成素(LH)等指标;②选用ICR雄性小鼠,随机分为正常对照组、模型组、男宝组(0.5 g.kg-1)五子衍宗丸高、中、低剂量组(2.4,1.2,0.6 g.kg-1)。除正常对照组外,各组均肌注氢化可的松造模(37.5 mg.kg-1),1次/日,连续7 d,造成小鼠肾阳虚模型,ig给药7 d。记录各组动物体重、体温、自主活动数及低温游泳存活时间。分别称量脾脏、胸腺及生殖器官质量并计算脏器指数。结果:五子衍宗丸组大鼠的扑捉次数、射精次数及附睾指数明显增加(P<0.05～P<0.01),T有升高的趋势、LH显著降低(P<0.01～P<0.001)。五子衍宗丸小鼠低温游泳存活时间明显延长(P<0.01～P<0.001),体温及自主活动数有升高趋势,脾脏及提肛肌指数有所升高(P<0.05),睾丸及附睾指数有增加的趋势。结论:提示五子衍宗丸具有补肾壮阳改善男性性功能低下的作用;并具有明显耐寒、抗疲劳,改善肾阳虚证候作用。

Objective： To observe the effect of Wuzi Yanzong Wan on the intercourse capacity of rats and the kidney-yang deficiency mice and investigate its pharmacological action of invigorating kidney and strengthening Yang. Method： （1）Male Wistar rats were randomly divided into the normal control group, Nanbao group （0.35 g·kg^-1 ） , Wuzi Yanzong Wan group （2.0, 1.0, 0.5 g ·kg^-1 ）. The rats were administrated daily by oral for 28 days. After the female rat was crated to the container with the male rat, the mating capability of male rat was evaluated by the catching latency, ejaculation latency, capture times and ejaculation frequency. Meanwhile, the content of testesterone （T）, and luteinizing hormone （LH） in rat serum were detected; （2）Male ICR mice were divided randomly into normal group, model group, Nanbao group （0.35 g·kg^-1）, Wuzi Yanzong Wan group （2.4, 1.2, 0.6 g ·kg^-1）. Except for the normal group, the other groups were treated by hydrocortisone for a week. At 7 days post treatment with drug, the weight, body temperature, the numbers of autonomic activity and the survival time of swimming with low temperature were recorded. The spleen, thymus and genitals were weight to calculate the viscera index. Result： Wuzi Yanzong Wan can increase the catching numbers, ejaculation frequency and epididymis index significantly （P 〈0.05-P 〈0.01 ）. The content of T in blood has a rising trend while the LH was decreased significantly （P 〈 0.01-P 〈 0. 001 ） compared to the control group. Wuzi Yanzong Wan can prolong its swimming endurance in low temperature （P 〈 0.01-0. 001 ） and elevate the body temperature and the numbersof autonomic activity. Besides, the spleen weight and he index of levator ani muscle were increased （P 〈 O. 05 ）. The spermary weight and the index of epididymis has a increasing trend. Conclustion： Wuzi Yanzong Wan have the function of building kidney and strengthening yang which can improve the male sexual function and have a significant effect on cold-resist and anti-fatigue. It was demonstrated that can improve the syndrome of kidney Yang deficiency.