妇炎康药液灌肠法药效学研究

Experimental Study on Pharmacodynamics of Fuyankang Enema

目的:观察妇炎康灌肠剂对小鼠抗炎、镇痛和免疫系统的影响。方法:①选用240只SPF级昆明种小鼠随机分为模型组,西药组(吲哚美辛0.025 g.kg-1.d-1),妇炎康ig高剂量组(2 g.kg-1.d-1),妇炎康灌肠高、中、低剂量组(2,1,0.5 g.kg-1.d-1)。以小鼠腹腔伊文斯蓝溶液吸光度、二甲苯致耳肿胀度和抑制率、热刺激痛阈和醋酸所致小鼠扭体次数和初次扭体时间为指标,观察妇炎康灌肠各组给药4 d对正常小鼠炎症抑制和痛阈的影响;②选用120只SPF级昆明种级小鼠,模型组代替西药组,余同①,以免疫抑制(0.025 g.kg-1.d-1醋酸泼尼松,ig 10 d)小鼠脾脏指数、胸腺指数以及廓清指数为指标,观察妇炎康灌肠各组给药10 d对免疫低下小鼠免疫系统的影响。结果:妇炎康灌肠剂各组能不同程度降低二甲苯所致小鼠耳廓肿胀度(P<0.05或P<0.01),提高小鼠痛阈(P<0.05),减少醋酸所致扭体次数(P<0.05或P<0.01),延迟初次扭体时间(P<0.05);抑制醋酸泼尼松造成的小鼠脏器系数减小(P<0.05或P<0.01)和巨噬细胞廓清指数减小(P<0.05)。结论:妇炎康灌肠给药有抗炎镇痛及免疫调节作用。

Objective： To study the pharmacodynamics on Fuyankang enema in treatment of chronic pelvic inflammatory disease, concerning anti-inflammatory, analgesic and immune. Method： Two hundred and forty Special-pathogen free KM rats were randomly divided into six groups： normal control group, western medicine group （ Indomethacin 0. 025 g·kg^-1·d^ -1 ） , high-dose Fuyankang ig group （ 2 g·k^g-1·d^-1） , low-dose, middledose, and high-dose Fuyankang enema groups （2, 1, 0.5 g·kg6-1·d^-1respectively）. Effects of Fuyankang enema on abdominal capillary permeability, ear edema, hot-plate tests and writhing were observed. Other 120 rats were divided in the same way, but western medicine group was replaced by model group. Immune organ relative weight and the charcoal clearance index were also observed in prednisone acetate induced immunodepressive rats. Result： Fuyankang enema had inhibitory effects on mice ear edema （P 〈 0.05 or P 〈 0.01 ） and reduction of immune organ relative weight （P 〈 0.05 or P 〈 0.01 ） and charcoal clearance index （P 〈 0.05 ） caused by prednisone had a reduction in ratio of writhing of mice （P 〈 0.05） and had a addition on pain threshold of mouse （P 〈 0.05 ）. Conclusion： Fuyankang enema has pharmaceutical effects in agreement with clinical effect on chronic pelvic inflammation, combination with oral medication is better.