心舒汤对动物心律失常和心肌缺血模型的保护作用

Protection of Xinshu Dicoction to Arrhythmia and Myocardial Ischemia Model

目的:研究心舒汤对模型大鼠、小鼠心律失常和心肌缺血的保护作用。方法:100只小鼠随机分成舒心汤高、中、低剂量组(1.7,0.85,0.42 g.kg-1),模型组(0.2 mL纯水)和冠心丹参胶囊组(0.35 g.kg-1),连续ig给药14 d后,复制三氯甲烷小鼠心律失常模型观察小鼠室颤发生率。大鼠50只随机分成5组,舒心汤高、中、低剂量组(1.18,0.59,0.29 g.kg-1),模型组(2 mL纯水)和冠心丹参胶囊组(0.24 g.kg-1)连续ig给药14 d,建立乌头碱致大鼠心律失常的模型,观察不同剂量舒心汤对模型动物心律失常的保护作用。60只大鼠随机分成舒心汤高、中、低剂量组,空白对照组、冠心丹参胶囊组和模型组,给药剂量、方法同上大鼠,空白对照组给药同模型组,连续给药14 d;12 d开始使用异丙肾上腺素建立大鼠心肌缺血模型,连续造模2 d,观察心舒汤对心肌缺血的保护作用。结果:心舒汤高剂量组使三氯甲烷导致的小鼠心室纤颤(VF)的发生率降低至25%;能增加导致的大鼠室性早搏(VP)、室性心动过速(VT)、室颤(VF)的乌头碱用量19.7,23.7,32.6μg.kg-1;减小ECG中T波位移幅度;使心肌缺血大鼠血清中肌酸激酶(CK)和谷草转氨酶(AST)含量降低到2 572.9,111.3 U.L-1。结论:心舒汤对心律异常和心肌缺血具有保护作用。

Objective： To investigate the protecting effect of Xinshu dicoction against arrhythmia and ischemia myocardial in animal model. Method： Hundred mice were divided into Xinshu dicoction high, middle and low dose group （1.7, 0.85, 0.42 g·kg^-1）, model group （0.2 mL water）, Guanxin Danshen capsules （0.35 g· kg^-l） group at random. After continuous oral administration for 14 d, arrhythmia animal model induced by chloroform in mice was copied to observe incidence of ventricular fibrillation （VF）. Fifty rats were randomly divided 5 groups： Xinshu dicoction high, middle and low dose group （1.18, 0.59, 0.29 g·kg^-1） , model group （2 mL water）, Guanxin Danshen capsules group （0.24g·kg^-1）. Oral administration lasted 14 days, isoproterenol-induced myocardial ischemia model was esyablished in rats to observed protective effect of Xinshu dicoction. Total 60 rats were divided into 6 groups and given drugs for 14 d in the same way as mentioned above. In the 12 d and isoproterenol （ISO） was used to establish myocardial ischemia model in rats twice. This was aimed to watch the protection of Xinshu dicoction on myocardial ischemia. Result： Xinshu dicoction could reduce the incidence of VF caused by chloroform to 25% in mice, could increase dosage of aconitine when ventricular prematoure （VP）, ventricular tachycardia （VT）, ventricular fibrillation （VF） happened in rats to 19.7,23.7, 32.6 μg ·kg^-1. Xinshu dicoction could reduce wave amplitude of T wave and the level of creatine kinase （CK） and aspartate aminotransiferase （AST） in serum of ischemia myocardial rats to 2 572.9, 111.3 U·L^-1 Conclusion： Xinshu dicoction has a protective effect on arrhythmia and ischemia myocardial.