红花黄色素对急性心肌缺血大鼠的保护作用

Protection Effect of Saffower Yellower in Acute Myocardial Ischemic Rats

目的:观察红花黄色素对急性心肌缺血大鼠心肌组织中丙二醛(MDA)、超氧化物歧化酶(SOD)的影响及对心肌组织损伤的保护作用。方法:大鼠分为5组,阳性药组(丹参注射液,含生药3.75 g.kg-1)、红花黄色素高、低剂量组(含生药80,40 mg.kg-1)、正常对照组(生理盐水)、模型组(生理盐水),每组13只,均ip,连续7 d,于第8天ip垂体后叶素(Pit)20 U.kg-1造模成大鼠急性心肌缺血模型,测定左心室心肌组织中SOD,MDA的变化以及观察心肌梗死面积的变化。结果:在造模成功的前提下,红花黄色素高、低剂量组与模型组比,可显著增加大鼠左心室心肌中SOD活性[(47.57±11.52)vs(25.97±7.73)U.mg-1,P<0.01;(41.28±11.97)vs(25.97±7.73)U.mg-1,P<0.05],并明显降低MDA含量[(0.29±0.08)vs(1.40±0.26)nmol.mg-1,P<0.01;(0.45±0.15)vs(1.40±0.26)nmol.mg-1,P<0.01],且呈量效关系;经TTC染色,红花黄色素高剂量组梗死区面积明显小于模型组[(32.20±2.2)%vs(43.84±6.3)%,P<0.05]。结论:红花黄色素注射液对Pit所致的急性心肌缺血大鼠有保护作用,其作用机制可能与抗氧化有关。

Objective： To investigate the regulatory effects of saffower yellower on superoxide dismutase （SOD）, malondialdehyed （MDA） and infarction area in acute myocardial ischemic rats. Method： Rats were randomly divided into 5 groups （n = 13 each） ： the controls and the model group （normal saline, ip）, the positive controls [ Danshen injection, 3.75 g .kg-~, ip） and Saffower Yellower high dose and low dose groups （80, 40 mg.·kg^-1ip）. The treatment lasted for 7 days respectively. On the 8th day, rats were injected with pituitrin （20 U·kg^-1, ip） to induce acute myocardial ischemia. Then the SOD activity and MDA concentration in heart tissues and heart infarction areas in rats were detected. Result： In comparison to group, modle saffower yellower 80 mg·kg^-1group significantly enhanced SOD activity [ （47.57±11.52） vs （25.97±7.73） U·mg^-1, P〈0.01]; [ （41.28±11.97） vs （ 25. 97± 7. 73 ） U·kg^-1 P〈0.05] and suppressed MDA concentration [ （0.29±0.08） vs （1.40±0.26） nmol ·mg^-1, P 〈0. 01 ] ; [ （0. 45 ±0. 15） vs （1.40 ±0. 26） nmol ·mg^-1 , P 〈0. 01 , in left ventricle tissues. The infarction areas were decreased in 80 mg ·kg^-1 saffower yellower group compared to model group [ （32. 20 ± 2.2） % vs （43.84±6. 3）% , P 〈 0. 05 ]. Saffower yellower dose dependently reversed these pathological changes significantly. Conclusion： Saffower yellower has protective effect to the pathological impairment in rats with acute myocardial ischemic induced by oituitrin. This is likely related to its anti-oxidation action.